Abstract: Provided herein are bispecific antigen-binding molecules that bind HER2 and methods of use thereof. The bispecific antigen-binding molecules comprise a first and a second antigen-binding domain, wherein the first and second antigen-binding domains bind to two different (preferably nonoverlapping) epitopes of the extracellular domain of human HER2. The bispecific antigen-binding molecules cluster on the surface of HER2 IHC2+ and IHC3+ cells, and are internalized into the cellular lysosomes. Also included are antibody-drug conjugates (ADCs) comprising the antibodies or bispecific antigen-binding molecules provided herein linked to a cytotoxic agent, radionuclide, or other moiety, as well as methods of treating cancer in a subject by administering to the subject a bispecific antigen-binding molecule or an ADC thereof.

Abstract: Whole milk and at least one additional nutrient source are admixed to reach a target diet and the amount of each of the admixture components is calculated based on compositional analysis results of the available whole milk, known levels of nutrients in the additional nutrient source, and the dietary target(s) for the young animals. The compositional analysis and admixing may be performed at the location where the young animals are fed.

Abstract: Whole milk and at least one additional nutrient source are mixed to reach an admixture of about 10 to about 17 percent total solids on a dry matter basis, where about 20 to about 26 percent of the total solids is protein, and where about 16 to about 25 percent of the total solids is fat. The admixture and a starter feed are fed to one or more young animals so that they receive at least 0.75 lbs. of total solids on a dry matter basis per day from the admixture. In response to ingesting the admixture, starter feed intake is increased and improved performance results. The level of the additional nutrient source(s) in the admixture may be calculated based on a compositional analysis of the available whole milk and a dietary target for the young animals. The compositional analysis may be performed at the location where the young animals are fed.