Abstract: The present invention is directed to methods for delivering stem cells to a target tissue in a mammal using glycoconjugate to traffic the stem cells to a desired organ in the mammal. The methods according to the present invention are especially applicable to administering stem cells such as those derived from the bone marrow or from umbilical cord tissue. The methods are also useful for targeting a gene of interest to a tissue in a mammal by introducing a cell containing the gene of interest and administering a glycoconjugate to the mammal.

Abstract: Blood collection systems include an integral flexible filter to remove leukocytes from blood components.

Abstract: A method of making a blood filter assembly provides first and second filter housing elements from a flexible thermoplastic material, each of the first and second housing elements including a molded port. The method places a filter media between the first and second filter housing elements and applies radio frequency heating and pressure to form a peripheral seal that joins the first and second filter housing elements to the filter media and encapsulates the filter media between the first and second housing elements.

Abstract: A filter for removing leukocytes from blood comprises a filter media enclosed in a housing. The filter media includes a main filter region comprising a porous membrane structure extending between first and second skin surfaces. The porous membrane structure is formed by intersecting cells having a range of diameters. The cells adjacent to the first skin surface have diameters generally smaller than the diameters of the cells adjacent to the second skin surface. The first skin surface includes an open area defined by pores, which are formed by the intersection of cells with the first skin surface. The majority of the open area is defined by pores having a diameter of between about 12 &mgr;m and 28 &mgr;m.

Abstract: The present invention is directed to methods for delivering cells to a target tissue in a mammal using glycoconjugate to traffic the cell to a desired organ in the mammal. The methods according to the present invention are especially applicable to administering stem cells such as those derived from the bone marrow or from umbilical cord tissue. The methods are also useful for targeting a gene of interest to a tissue in a mammal by introducing a cell containing the gene of interest and administering a glycoconjugate to the mammal.

Abstract: A fluid filter assembly for filtering fluids such as blood is described. The assembly includes first and second filter housing elements formed by an injection molding process. Each element is flexible and includes a peripheral flange formed thereabout and a fluid communicating port formed therein. Filter media, such as a filter membrane, is sealed between the mating flanges of two elements. The fluid filter assembly is capable of collapsing and expanding during the filtration process depending upon the composition of the fluid passed there through. A method for making the filter assembly and systems for using the filter assembly are also disclosed.

Abstract: A filter for removing leukocytes from blood comprises a filter media enclosed in a housing. The filter media includes a main filter region comprising a porous membrane structure extending between first and second skin surfaces. The porous membrane structure is formed by intersecting cells having a range of diameters. The cells adjacent to the first skin surface have diameters generally smaller than the diameters of the cells adjacent to the second skin surface. The first skin surface includes an open area defined by pores, which are formed by the intersection of cells with the first skin surface. The majority of the open area is defined by pores having a diameter of between about 12 &mgr;m and 28 &mgr;m.

Abstract: Blood collection systems include an integral flexible filter to remove leukocytes from blood components.

Abstract: Blood collection systems include an integral flexible filter to remove leukocytes from blood components.

Abstract: The invention provides a leukodepletion filter including a plurality of glass fiber filter pads, and a plurality of non-woven polyester fiber filter pads, sealed within a filter housing.

Abstract: A tyrosine-substituted hirudin analog has antithrombogenic activity. Further simultaneous reaction at native tyrosine residues is prevented by mutation at those sites to encode nonreactive amino acids. Several novel strategies for coupling the hirudin analog to solid surfaces while simultaneously conserving antithrombogenic activity are disclosed.

Abstract: A leukodepletion filter is provided including glass fiber filter pads and non-woven polyester fiber filter pads sealed within a filter housing. The filter is designed such that a fluid, such as whole blood, packed red blood cells, platelets or plasma, is conducted through the glass fiber filter pads before the non-woven polyester fiber filter pads. A method is further provided for leukodepleting a body fluid by filtering the body fluid through glass fiber filter pads and also filtering the body fluid through non-woven polyester fiber filter pads. After filtering, the body fluid is collected.

Abstract: An anhydrous solid water-soluble composition and method for direct controlled administration of beneficial agents to a flowing medical fluid. The beneficial agents are geometrically dispersed in a solid matrix and isolated from one and other to allow administration of mutually reactive agents.

Abstract: Immunoassays and nucleic acid assays are provided along with liquid perfluorocarbon supports utilized in these methods. The support is based on an inert perfluorocarbon carrier with ligands or binders for the ligands attached to its surface through a highly fluorinated isocyanate anchor group.

Abstract: Liquid perfluorocarbon supports useful as liquid affinity supports are provided. The support is based on an inert perfluorocarbon carrier with ligands or binders for the ligands attached to its surface through a highly fluorinated isocyanate anchor group.

Abstract: Bioaffinity and ion-exchange separation methods are provided along with liquid supports utilized in these methods. The support is based on an inert carrier with ligands or binders attached to its surface through a highly fluorinated isocyanate anchor group. Methods for preparing such supports and their use in capturing neutral and charged target molecules from samples and in analytical applications are also provided.

Abstract: Methods for preparing liquid supports utilized in bioaffinity and ion-exchange separation methods and provided. The support is based on an inert carrier with ligands or binders for ligands attached through its surface through a highly fluorinated isocyanate anchor group. The use of such supports in capturing neutral and charged target molecules from samples and in analytical applications are also provided.

Abstract: An adherent cell culture flask is provided. The flask includes a body constructed from a first side wall and a second side wall, each side wall having an interior surface and an exterior surface. The first and second side walls are sealed together to define a chamber. The first side wall is constructed from flexible film and includes on its interior surface a charged surface for facilitating the attachment of cells thereto. The second side wall is constructed from flexible film and includes a non-smooth interior surface for preventing the first side wall interior surface from sticking to the second side wall interior surface.

Abstract: A blood separation centrifuge rotor having a generally parabolic core disposed concentrically and spaced apart within a housing having a similarly shaped cavity. Blood is introduced through a central inlet and into a central passageway enlarged downwardly to decrease the velocity of the entrant blood. Septa are disposed inside the central passageway to induce rotation of the entrant blood. A separation chamber is defined between the core and the housing wherein the whole blood is separated into red cell, white cell, and plasma zones. The zones are separated by annular splitter blades disposed within the separation chamber. The separated components are continuously removed through conduits communicating through a face seal to the outside of the rotor.