Abstract: A bioprocess apparatus is disclosed that is capable of efficiently disconnecting large bioprocess tubes in a bioprocess system. The bioprocess apparatus includes a separating collar made from a malleable and rigid material that can be placed over large bioprocess tubes. In one aspect, the separating collar includes a pair of adjacent separating edges that allow the collar to be slidably mounted onto a bioprocess tube. Once positioned on the bioprocessed tube, a cutting operation takes place that goes through the separating collar and the bioprocess tube resulting in a sterile disconnect.