Abstract: Disclosed herein are tricyclic compounds, including pyrimido[4?,5?:4,5]thieno[2,3-c]pyridazine-8-amine, pyrido[3?,2?:4,5]thieno[3,2-d]pyrimidine-4-amine, pyrazino[2?,3?:4,5]thieno[3,2-d]pyrimidin-4-amine, pyrido[3?,2?:4,5]furo[3,2-d]pyrimidin-4-amine, and pyrimido[4?,5?:4,5]furo[2,3-c]pyridazin-8-amine compounds, which may be useful as positive allosteric modulators of the muscarinic acetylcholine receptor M4 (mAChR M4). Also disclosed herein are methods of making the compounds, pharmaceutical compositions comprising the compounds, and methods of treating neurological and psychiatric disorders associated with muscarinic acetylcholine receptor dysfunction using the compounds and compositions.

Abstract: A medium for preserving and extending the viable useful life of poultry (e.g., turkey) semen, in vitro, is provided. Semen is preserved without loss of fertility for up to about 24 hours in the medium. The medium contains di-potassium phosphate, sodium glutamate, fructose, sodium acetate, mono-potassium phosphate, potassium citrate, magnesium chloride, N-tris hydroxymethyl methyl-2-aminoethane sulfonic acid, phosphatidylcholine, and zinc, and optionally sialic acid, carnitine, serine ethanolamine phosphodiesterase, or mixtures thereof. A method for extending the fertility of poultry (e.g., turkey) semen for up to about 24 hours when held in vitro at about 5° to about 10° C., involving mixing poultry (e.g.