Abstract: Provided is a microfluidic mixing system comprising a loop system for transferring one or more fluids, wherein the loop system comprises a plurality of sub loops, each sub loop formed from one or more common channels shared with at least one other sub loop and completed by an outer channel portion that is not shared by any other sub loop, and wherein the outer channel portion of each sub loop comprises one or more valves such that the sub loop is capable of isolation from all other sub loops and each common channel comprises one or more valves such that the common channel is capable of isolation from the remainder of the loop system, and wherein one or more sub loops in the system comprise valves that are configured to enable peristaltic mixing.

Abstract: A microfluidic system comprising a 1st reaction zone, a 2nd reaction zone, a reagent delivery channel configured to deliver one or more reagents to the 1st reaction zone, a waste channel to remove waste from the 2nd reaction zone, a 1st sample delivery channel configured to deliver a sample to the 1st reaction zone and a 2nd sample delivery channel configured to deliver a sample to the 2nd reaction zone; wherein the microfluidic system comprises a means for retaining one or more reagents in each reaction zone; and wherein the 1st reaction zone and 2nd reaction zone are connected in series by a reaction zone channel.