Abstract: A bioprinter for fabricating three-dimensional (3D) cell constructs, the bioprinter comprising one or more holding reservoirs for holding a fluid sample; a printstage for holding a sample container and supporting a substrate on which a 3D cell construct is to be printed; a sample loading system in fluid communication with the one or more holding reservoirs, the sample loading system configured to load a sample from a sample container into the one or more holding reservoirs; a pump in fluid communication with the sample loading system, the pump configured to draw the sample out of a sample container and pump the sample into the one or more holding reservoirs; and a droplet dispensing system in fluid communication with the one or more reservoirs, the droplet dispensing system configured to print sample droplets from the one or more reservoirs onto a substrate supported by the printstage.

Abstract: A process for producing a 3D tissue culture model by (a) printing a drop of bio-ink to a substrate; (b) printing a drop of activator to the drop of bio-ink to form a hydrogel droplet; (c) repeating steps (a) and (b) in any order to form a hydrogel mold adapted to receive a drop containing cells; (d) printing a drop containing cells to the hydrogel mold; and (e) repeating steps (a) and (b) in any order to form a 3D tissue culture model comprising the cells encapsulated in the hydrogel mold.

Abstract: A process for producing a 3D tissue culture model by (a) printing a drop of bio-ink to a substrate; (b) printing a drop en.) of activator to the drop of bio-ink to form a hydrogel droplet; (c) repeating steps (a) and (b) in any order to form a hydrogel mold adapted to receive a drop containing cells; (d) printing a drop containing cells to the hydrogel mold; and (e) repeating steps (a) and (b) in any order to form a 3D tissue culture model comprising the cells encapsulated in the hydrogel mold.