Abstract: The invention relates to mutant G genes of viral haemorrhagic septicaemia virus (VHSV) in rainbow trout, which encode mutant G proteins of VHSV with defective or null binding to cells that can be infected by VHSV. Said mutant G genes of VHSV can be used, among other applications, to produce vaccines (DNA vaccines or attenuated live vaccines) for preventing disease caused by VHSV in animals that can be infected by VHSV, generating non-human transgenic animals, and developing reagents for the diagnosis of infection caused by VHSV.

Abstract: The gene construct comprises a nucleic acid sequence that encodes an immunogenic peptide of an aquatic animal pathogen under the control of an expression control sequence that can direct the expression of said immunogenic peptide in an aquatic animal and which comprises the sequence of a cytomegalovirus (CMV) promoter joined to an expression-enhancing sequence at position 5?. The vector comprises said gene construct and can be used to produce DNA vaccines that can be used to prevent infectious diseases in aquatic animals and is of particular interest in aquaculture. Said vaccines can be used for the mass vaccination of aquatic animals using an immersion/sonication process.