Abstract: The present disclosure pertains to a microfluidic platform. The microfluidic platform includes a top layer having a top inlet and outlet, a center layer having a center inlet and outlet, and a bottom layer having a bottom inlet and outlet. The microfluidic platform further includes a first porous membrane between the top and center layer, a second porous membrane between the center and bottom layer, a first electrode disposed on at least one of the top and bottom layers, and a second electrode disposed on at least one of the top and bottom layers. Additionally, the present disclosure pertains to a method for selective isolation. The method includes flowing a sample through a microfluidic platform, isolating a first component from the sample in a top layer, isolating a second component from the sample in a center layer, and isolating a third component from the sample in a bottom layer.

Abstract: A system for detecting molecular structures is provided. The system includes a radio frequency (RF) wireless antenna, batteryless sensor, and a network analyzer. The sensor has an ultra-high frequency dipole antenna that transmits data to the RF wireless antenna and a sensing element. The sensing element is operatively coupled to the dipolar antenna and detects molecular structures. The network analyzer receives data from the RF wireless antenna and analyzes the data to determine a concentration of molecular structures.

Abstract: This disclosure describes a multiplexing, wireless, batteiyless multiplexing paper sensor. The sensor includes a polymeric, non-cellulosic substrate that includes a radio frequency (RF) wireless communication ultra-wide band (UWB) antenna and a sensing element disposed on the polymeric, non-cellulosic substrate with and without molecular imprinted structures to immobilize specific target chemical or biological objects. The sensor is powered when a signal is received by the RF wireless communication UWB antenna and then takes measurements of a medium being monitored by the sensor. Data corresponding to the measurements is then transmitted from the sensor via the RF wireless communication UWB antenna.

Abstract: Hydrogel-based sorbents and methods for their use in collecting, concentrating, and removing environmental per- and poly-fluoroalkyl substances. In one aspect, the invention provides hydrogel-based sorbents that are effective for collecting, concentrating, and removing PFASs from an environment in which the sorbent is placed; an environment in which the sorbent is in contact with (e.g., liquid communication).

Abstract: There is a need in the point-of-care diagnostic community for an efficient and portable method for testing blood and other biological fluids that can be easily translated across multiple applications. An aspect of the invention described involves monitoring the optical properties of molecularly-mediated nanoparticle assemblies though an optically transparent and magnetically active microfluidic chip, which has recently emerged as an attractive method for biomarker detection as it is an efficient tool for monitoring the binding events that take place in a sensing assay. In one embodiment, this device is directed towards two-nanoparticle assays that rely on the assembly or disassembly of plasmonic and magnetic nanoparticles in response to a certain analyte. A further embodiment is directed to a spiral microfluidic using inertial forces to filter fluid components by size, connected to a magnetically active channel comprised of a nickel micropad array, optically transparent microchannel, and permanent magnets.

Abstract: Provided herein are methods and compositions for the detection and standard free quantitation of antigens of interest. Further provided are methods for the absolute quantitation of proteins in solution, cells, or tissues. Also provided are methods for determining the on-target binding constant of a therapeutic agent to its target in a biological sample.

Abstract: There is a need in the point-of-care diagnostic community for an efficient and portable method for testing blood and other biological fluids that can be easily translated across multiple applications. An aspect of the invention described involves monitoring the optical properties of molecularly-mediated nanoparticle assemblies though an optically transparent and magnetically active microfluidic chip, which has recently emerged as an attractive method for biomarker detection as it is an efficient tool for monitoring the binding events that take place in a sensing assay. In one embodiment, this device is directed towards two-nanoparticle assays that rely on the assembly or disassembly of plasmonic and magnetic nanoparticles in response to a certain analyte. A further embodiment is directed to a spiral microfluidic using inertial forces to filter fluid components by size, connected to a magnetically active channel comprised of a nickel micropad array, optically transparent microchannel, and permanent magnets.

Abstract: There is a need in the point-of-care diagnostic community for an efficient and portable method for testing blood and other biological fluids that can be easily translated across multiple applications. An aspect of the invention described involves monitoring the optical properties of molecularly-mediated nanoparticle assemblies though an optically transparent and magnetically active microfluidic chip, which has recently emerged as an attractive method for biomarker detection as it is an efficient tool for monitoring the binding events that take place in a sensing assay. In one embodiment, this device is directed towards two-nanoparticle assays that rely on the assembly or disassembly of plasmonic and magnetic nanoparticles in response to a certain analyte. A further embodiment is directed to a spiral microfluidic using inertial forces to filter fluid components by size, connected to a magnetically active channel comprised of a nickel micropad array, optically transparent microchannel, and permanent magnets.

Abstract: There is a need in the point-of-care diagnostic community for an efficient and portable method for testing blood and other biological fluids that can be easily translated across multiple applications. An aspect of the invention described involves monitoring the optical properties of molecularly-mediated nanoparticle assemblies though an optically transparent and magnetically active microfluidic chip, which has recently emerged as an attractive method for biomarker detection as it is an efficient tool for monitoring the binding events that take place in a sensing assay. In one embodiment, this device is directed towards two-nanoparticle assays that rely on the assembly or disassembly of plasmonic and magnetic nanoparticles in response to a certain analyte. A further embodiment is directed to a spiral microfluidic using inertial forces to filter fluid components by size, connected to a magnetically active channel comprised of a nickel micropad array, optically transparent microchannel, and permanent magnets.

Abstract: The present invention includes a mold and a method for producing an engineered tissue construct comprising: providing a mold comprising one or more openings, the mold being at least partially elastic and the one or more openings having a pre-determined shape; and extruding through the one or more openings in the mold a biocompatible gel-forming macromer to form a hydrogel using a mechanical force sufficient to extrude the biocompatible gel-forming macromer.

Abstract: Nanofibers are formed using electrospray deposition from microfluidic source. The source is brought close to a surface, and scanned in one embodiment to form oriented or patterned fibers. In one embodiment, the surface has features, such as trenches on a silicon wafer. In further embodiments, the surface is rotated to form patterned nanofibers, such as polymer nanofibers. The nanofibers may be used as a mask to create features, and as a sacrificial layer to create nanochannels.

Abstract: A single molecule or molecule complex detection method is disclosed in certain aspects, comprising nano- or micro-fluidic channels.

Abstract: Nanofibers are formed using electrospray deposition from microfluidic source. The source is brought close to a surface, and scanned in one embodiment to form oriented or patterned fibers. In one embodiment, the surface has features, such as trenches on a silicon wafer. In further embodiments, the surface is rotated to form patterned nanofibers, such as polymer nanofibers. The nanofibers may be used as a mask to create features, and as a sacrificial layer to create nanochannels.

Abstract: A single molecule or molecule complex detection method is disclosed in certain aspects, comprising nano- or micro-fluidic channels.

Abstract: Nanofibers are formed using electrospray deposition from microfluidic source. The source is brought close to a surface, and scanned in one embodiment to form oriented or patterned fibers. In one embodiment, the surface has features, such as trenches on a silicon wafer. In further embodiments, the surface is rotated to form patterned nanofibers, such as polymer nanofibers. The nanofibers may be used as a mask to create features, and as a sacrificial layer to create nanochannels.

Abstract: A nanofiber membrane is formed on a microfiber membrane. The nanofiber membrane may be electro sprayed directly onto the microfiber membrane and becomes integrated with the microfiber membrane to form a filter. The microfiber membrane provides structural integrity to for the nanofiber membrane, and an additional microfiber membrane may be added to sandwich the nanofiber membrane.

Abstract: A nano-fluidic trapping device and method of fabrication are disclosed. In one embodiment, a nano-fluidic trapping device for assembling a SERS-active cluster includes a substrate. The nano-fluidic trapping device further includes a SERS-active cluster compartment. The SERS-active cluster is formed in the SERS-active cluster compartment. In addition, the nano-fluidic trapping device includes a reservoir. The reservoir allows introduction of target molecules into the nano-fluidic trapping device. Moreover, the nano-fluidic trapping device includes a microchannel. The microchannel allows the target molecules to be introduced to the SERS-active cluster compartment from the reservoir. The nano-fluidic trapping device also includes a nanochannel. The SERS-active cluster compartment, the reservoir, the microchannel, and the nanochannel are disposed within the substrate.

Abstract: Nanofibers are formed using electrospray deposition from microfluidic source. The source is brought close to a surface, and scanned in one embodiment to form oriented or patterned fibers. In one embodiment, the surface has features, such as trenches on a silicon wafer. In further embodiments, the surface is rotated to form patterned nanofibers, such as polymer nanofibers. The nanofibers may be used as a mask to create features, and as a sacrificial layer to create nanochannels.

Abstract: A fiber optic multiple-pass surface plasmon resonance technique provides an increase in the number of passes to any arbitrary number is described. Multiple reflections off a reflective sample surface are achieved in one embodiment using a fiber optic collimator, a reflector, and a second reflector, such as a corner cube prism. An electric field assist may be provided by migrating charged molecules to be detected toward the reflective sample surface. In further embodiments, the filed assist may be used with a single pass surface plasmon resonance technique. In still further embodiments, an electo-optic modulated recirculation loop may be used to increase the number of reflections off the sample surface.

Abstract: A system and method for detecting changes in the refractive index of a fluid in a small test volume. A change in the refractive index can indicate a change in the chemical composition of the fluid. The test volume has a depth comparable to or less than the wavelength of incident light. In one embodiment, an internal surface of the volume is coated with a binding partner selected to bind with a targeted molecule. When the targeted molecule binds to the binding partner, the optical properties of the system change. The refractive index is determined by illuminating the test volume with laser light and measuring transmitted or reflected light.