Abstract: A method prepares data for analysis. A user interface includes a flow pane, which includes a flow diagram with nodes, each node having an intermediate set and specifying an operation. A first user input selects a first node, which specifies a first operation. In response, the method displays node internals for the first node, including a description of the first operation. A second user input combines a second node with the first node. The second node specifies a second operation. In response to the second user input, the method (i) removes the second node from the flow diagram, (ii) determines ordering for the first and second operations, (iii) updates the node internals for the first node to specify the first and second operations, ordered according to the ordering, and (iv) displays the updated node internals, including descriptions of the first and second operations, sequenced according to the determined ordering.

Abstract: Contemporary gene sequencing techniques, including “Next Generation Sequencing” techniques, can include sequencing a plurality of fragments of a target polynucleotide. However, tire limitations of existing sequencing techniques, and the often repetitive or otherwise difficult-to-sequence structure of natural polynucleotides, means that it can be difficult and/or expensive to generate accurate sequences. Methods provided herein include inserting dual polynucleotide ‘barcodes,’ along with neighboring primer sequences, into a target polynucleotide prior to other sequencing processes. These inserted barcodes can improve the accuracy of sequences generated for the target by adding ‘noise’ into the target, allowing subsequent sequencing techniques (e.g., alignment, stitching, etc.) to more accurately estimate the target-plus-barcodes sequence.