Abstract: The method for producing a cell aggregate including glial progenitor cells according to the present invention comprises: (1) a step of subjecting pluripotent stem cells to suspension culture in an embryoid-body-forming culture medium containing one or more SMAD signaling inhibitors and one or more Wnt signaling activators in the absence of feeder cells for 5 days to 10 days, to form a cell aggregate; (2) a step of subjecting the cell aggregate obtained in (1) to suspension culture in an embryoid-body-forming culture medium containing retinoic acid; (3) a step of subjecting the cell aggregate obtained in (2) to suspension culture in an embryoid-body-forming culture medium or neuron-and-glia-proliferating culture medium containing retinoic acid and one or more SHH signaling activators; and (4) a step of subjecting the cell aggregate obtained in (3) to suspension culture in a neuron-and-glia-proliferating culture medium containing no retinoic acid and one or more SHH signaling activators.

Abstract: The purpose of the present invention is to provide detection methods of Notch signaling activation for detecting the activation of the Notch signaling in living cells simply and conveniently. The expression of a fluorescent protein Venus in a transgenic cell into which a vector having the fluorescent protein Venus gene which is controlled by the wild-type Hes-1 gene promoter has been introduced is compared with the expression of a fluorescent protein Venus in a transgenic cell into which a vector having the fluorescent protein Venus gene controlled by a mutated Hes-1 gene promoter which is not controlled by an activated Notch protein has been introduced, and a transgenic cell in which a signal by the expression of Venus introduced by the vector having the wild-type Hes-1 promoter is observed and in which a signal by the expression of Venus introduced by the vector having the mutated Hes-1 gene promoter which is not controlled by the activated Notch protein is not observed is identified.

Abstract: The purpose of the present invention is to provide detection methods of Notch signaling activation for detecting the activation of the Notch signaling in living cells simply and conveniently. The expression of a fluorescent protein Venus in a transgenic cell into which a vector having the fluorescent protein Venus gene which is controlled by the wild-type Hes-1 gene promoter has been introduced is compared with the expression of a fluorescent protein Venus in a transgenic cell into which a vector having the fluorescent protein Venus gene controlled by a mutated Hes-1 gene promoter which is not controlled by an activated Notch protein has been introduced, and a transgenic cell in which a signal by the expression of Venus introduced by the vector having the wild-type Hes-1 promoter is observed and in which a signal by the expression of Venus introduced by the vector having the mutated Hes-1 gene promoter which is not controlled by the activated Notch protein is not observed is identified.