Patents by Inventor Jun Ohgane

Jun Ohgane has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20100120036
    Abstract: To measure the methylation degree of a genomic DNA simply and correctly as well as exhaustively, there is provided a method for amplifying a DNA fragment, characterized by comprising the following steps (1) to (5): (1) digesting a sample double-stranded DNA with a first restriction enzyme; (2) adding a double-stranded DNA fragment consisting of a first oligonucleotide and a second oligonucleotide to a restriction enzyme cleavage end of the double-stranded DNA fragment produced in step (1) to obtain a DNA fragment; (3) digesting the DNA fragment obtained in step (2) with a second restriction enzyme; (4) allowing the double-stranded DNA fragment produced in step (3) to coexist with a third oligonucleotide and a fourth oligonucleotide in the presence of a ligase; and (5) heating the double-stranded DNA fragment obtained in step (4), thereby dissociating the double-stranded DNA fragment and then performing a polymerase chain reaction to selectively amplify a DNA fragment having the cleavage end of the first rest
    Type: Application
    Filed: March 5, 2008
    Publication date: May 13, 2010
    Inventors: Kunio Shiota, Shintaro Yagi, Yoko Takahashi, Satoshi Tanaka, Jun Ohgane
  • Publication number: 20060134659
    Abstract: It is an object of the present invention to provide a demethylation method that, from among multiple methylated regions present in the genome DNA of a living cell, can demethylate a specified methylated region only without influencing the other methylated regions, and to achieve the object, a target methylated region present in either a sense or antisense DNA chain in genome DNA of a living cell is demethylated, by artificially generating in the living cell the following (a) RNA comprising a base sequence equivalent to the base sequence of a region comprising all or part of the target methylated region in the DNA chain where the target methylated region is present, or (b) RNA comprising a base sequence equivalent to the base sequence of a region comprising a region neighboring the target methylated region in the DNA chain where the target methylated region is present.
    Type: Application
    Filed: September 6, 2005
    Publication date: June 22, 2006
    Applicant: TOUDAI TLO, Ltd.
    Inventors: Kunio Shiota, Satoshi Tanaka, Jun Ohgane, Takuya Imamura, Naka Hattori, Koichiro Nishino, Soshi Yamamoto, Naoko Hattori
  • Publication number: 20020072059
    Abstract: The present invention provides a method of identifying a cell, tissue or nucleus, comprising collecting information on the methylation pattern of DNA isolated from the cell, tissue or nucleus and analyzing the resultant information.
    Type: Application
    Filed: June 18, 2001
    Publication date: June 13, 2002
    Applicant: The University of Tokyo
    Inventors: Kunio Shiota, Satoshi Tanaka, Jun Ohgane, Naka Hattori