Abstract: Histamine may be quantitatively measured by providing the steps of: holding in a recess formed at the bottom of a vessel an oocyte that expresses histamine receptors, inserting first and second electrodes into the oocyte, measuring the membrane potential of the oocyte by means of said first electrode to stabilize the membrane potential of said oocyte at a predetermined level by driving a current through second electrode inserted into said oocyte by means of a circuitry for clamping membrane potential of oocyte, infusing a sample into a fine reacting tube having an antigen immobilized on the inner surface thereof together with some buffer solution to promote a histamine releasing reaction, transferring the solution containing histamine released in the fine reacting tube to the vessel to make contact with said oocyte in the vessel, detecting an electric response of oocyte caused by the contact with said solution, and determining the concentration of histamine released by said histamine releasing reaction in sai

Abstract: Histamine may be quantitatively measured by providing the steps of: holding in a recess formed at the bottom of a vessel an oocyte that expresses histamine receptors, inserting first and second electrodes into the oocyte, measuring the membrane potential of the oocyte by means of said first electrode to stabilize the membrane potential of said oocyte at a predetermined level by driving a current through second electrode inserted into said oocyte by means of a circuitry for clamping membrane potential of oocyte, infusing a sample into a fine reacting tube having an antigen immobilized on the inner surface thereof together with some buffer solution to promote a histamine releasing reaction, transferring the solution containing histamine released in the fine reacting tube to the vessel to make contact with said oocyte in the vessel, detecting an electric response of oocyte caused by the contact with said solution, and determining the concentration of histamine released by said histamine releasing reaction in sai

Abstract: An automatic electrophysiological measuring apparatus to automatically penetrate a glass electrode(s) into the membrane of a Xenopus Oocyte to hold the membrane potential and to automatically measure reactions to the administration of a medicine is to be provided.

Abstract: Histamine may be quantitatively measured by providing the steps of: holding in a recess formed at the bottom of a vessel an oocyte that expresses histamine receptors, inserting first and second electrodes into the oocyte, measuring the membrane potential of the oocyte by means of said first electrode to stabilize the membrane potential of said oocyte at a predetermined level by driving a current through second electrode inserted into said oocyte by means of a circuitry for clamping membrane potential of oocyte, infusing a sample into a fine reacting tube having an antigen immobilized on the inner surface thereof together with some buffer solution to promote a histamine releasing reaction, transferring the solution containing histamine released in the fine reacting tube to the vessel to make contact with said oocyte in the vessel, detecting an electric response of oocyte caused by the contact with said solution, and determining the concentration of histamine released by said histamine releasing reaction in sai

Abstract: Histamine may be quantitatively measured by performing the following steps. First, an oocyte that expresses histamine receptors is held in a recess formed at the bottom of a vessel. Then, first and second electrodes are inserted into the oocyte. Subsequently, the membrane potential of the oocyte is measured by using the first electrode to stabilize this membrane potential at a predetermined level by driving a current through the second electrode using circuitry for clamping the membrane potential of the oocyte. A sample is then infused into a fine reacting tube having an antigen immobilized on its inner surface together with some buffer solution to promote a histamine releasing reaction. The solution containing histamines that is released in the fine reacting tube is transferred to the vessel to make contact with the oocyte in the vessel.