Abstract: A gas pressure maintaining and adjusting device, a microstructure optical fiber and a preparation method of the microstructure optical fiber belong to the field of preparation of special optical fibers. In the gas maintaining and adjusting device, a communication control module is electrically connected with a main console of an optical fiber drawing tower; a signal output end of the communication control module is connected with a signal receiving end of a programmable logic controller (PLC); the PLC is provided with a gas pressure threshold display screen; the signal receiving end of the PLC is further connected with a signal output end of a pressure controller; and the PLC is further connected with an electromagnetic valve used for controlling opening and closing of a gas inlet and a gas outlet.