Abstract: A device for evaluating the neurovirulence of a mumps virus, comprising: (I) a virus inoculation module, which is used for performing virus inoculation of a mumps virus to be evaluated on the lateral ventricle of a rat; (II) a processing module, which is used for performing vibration slicing on the fixed rat brain; (III) an imaging module, which is used for scanning and imaging the obtained rat brain slices; and (IV) an analysis module, which is used in the obtained imaging for calculating a neurovirulence index by using a formula I: the neurovirulence index=S1/S0×100 (formula I) according to the cross-sectional area S1 of a cavity formed by hydrocephalus in the longitudinal section of the rat brain and the total cross-sectional area S0 of the rat brain. Multiple results show that the results are stable, repeatability is high, and a wild strain may be distinguished from a vaccine strain.

Abstract: Provided are an F-genotype mumps virus attenuated strain, a construction method therefor and an application thereof. The attenuated strain is a mumps virus with the accession number of CCTCC NO: V201950. Further provided are a vaccine composition containing the F-genotype mumps virus attenuated strain as an active ingredient and a preparation method thereof.