Abstract: The present disclosure provides a fiber laser light coherent combination system, comprising: a modulator module configured to perform a phase modulation on sub-beams according to pseudo-random sequences orthogonally independent from each other, and perform a frequency shift on a reference beam according to a set frequency; a fiber laser light amplifier module configured to perform a power amplification on the modulated sub-beams; a laser light collimation emission module configured to collimate and output the sub-beams and the reference beam; a combination sampling module configured to perform a combination of the sub-beams and the reference beam which are collimated and outputted, and convert them into an electrical signal; a digital phase modulation and demodulation module configured to perform a demodulation on the electrical signal according to the shifted frequency and each of the pseudo-random sequences, and obtain a phase difference between each of the sub-beams and the reference beam.

Abstract: The present disclosure relates to managing transmissions across multiple time-domain resources, including receiving, by a User Equipment (UE) from a network, one or more of at least one frequency-domain starting position for a transmission, at least one frequency resource set for the transmission, or at least one frequency hopping configuration set for the transmission. The UE determines a plurality of time-domain resources for repeating the transmission or for multiple transmissions scheduled by a same scheduling command. The UE determines at least one of a frequency-domain starting position, a frequency-domain resource set, or a frequency hopping configuration set for the transmission in each of the plurality of time-domain resources.

Abstract: Methods, apparatus, and systems that facilitate the indication of the codebook to be transmitted/retransmitted are disclosed. In one example aspect, a method for wireless communication includes transmitting, by a base station, a physical-layer control signaling message to a user device triggering a retransmission of a codebook for a feedback transmission that has been previously canceled. The physical-layer control signaling message includes a field of resource assignment that is used to indicate an attribute associated with the codebook. The method also includes receiving, by the base station, the retransmission of the codebook from the user device according to the physical-layer control signaling message.

Abstract: Therapeutic extracellular vesicles (EVs) containing high copies of functional nucleic acids and other biomolecules are produced in large quantities by laying donor cells on a surface of a chip, adding various plasmids, other transfection vectors and their combinations to a buffer on the chip, applying a pulsulatic electric field across the cells laid on top of the chip surface and plasmids/vectors buffer solution below the chip surface, and collecting the EVs secreted by the transfected cells. The chip surface has a three-dimensional (3D) nanochannel electroporation (NEP) biochip formed on it, capable of handling large quantities of the donor cells. The buffer is adapted for receiving plasmids and other transfection vectors.

Abstract: Therapeutic extracellular vesicles (EVs) containing high copies of functional nucleic acids and other biomolecules are produced in large quantities by laying donor cells on a surface of a chip, adding various plasmids, other transfection vectors and their combinations to a buffer on the chip, applying a pulsulatic electric field across the cells laid on top of the chip surface and plasmids/vectors buffer solution below the chip surface, and collecting the EVs secreted by the transfected cells. The chip surface has a three-dimensional (3D) nanochannel electroporation (NEP) biochip formed on it, capable of handling large quantities of the donor cells. The buffer is adapted for receiving plasmids and other transfection vectors.

Abstract: Devices for high throughput cell electroporation include a trapping component that at least partially defines an upper boundary of a microfluidic chamber. A cell trap array is patterned on the underside of the trapping component, and a channeling component is positioned beneath the trapping component. The channeling component includes a vertically oriented nanochannel array. The trapping component and the channeling component are positioned such that a given nanochannels is positioned beneath a cell trap. During use, fluid flow holds trapped cells in secure contact with the nanochannels beneath the cell trap. The device further includes upper and lower electrode layers for generating an electric field to electroporate trapped cells via the nanochannel array. A reservoir positioned beneath the channeling component can be filled transfection reagent solution. During electroporation, the transfection reagent solution travels through the nanochannel array during to transfect the trapped cells.

Abstract: Disclosed are peptides that contain up to about 35 amino acids, including a plurality of aromatic amino acid residues and either (i) an amino acid residue that is phosphorylated or sulfated, or (ii) an amino acid comprising an ester-moiety linked via peptide bond, or both (i) and (ii), wherein the peptide is capable of self-assembly to form nanofibrils in the presence of an enzyme that hydrolyzes the phosphate group, the sulfate group, or the ester-moiety. These peptides are enzymatically responsive hydrogelators, and they can be used to form pericellular hydrogels/nanofibrils upon exposure to target cells that secrete or express a surface bound ectoenzyme having hydrolase activity suitable to induce peptide gelation. These materials, and compositions containing the same, can be used for in vitro and in vivo cellular imaging, treating cancerous conditions, collecting a secretome from a cell upon which the pericellular hydrogels/nanofibrils form, and screening the collected secretome.

Abstract: The present disclosure provides a fiber laser light coherent combination system, comprising: a modulator module configured to perform a phase modulation on sub-beams according to pseudo-random sequences orthogonally independent from each other, and perform a frequency shift on a reference beam according to a set frequency; a fiber laser light amplifier module configured to perform a power amplification on the modulated sub-beams; a laser light collimation emission module configured to collimate and output the sub-beams and the reference beam; a combination sampling module configured to perform a combination of the sub-beams and the reference beam which are collimated and outputted, and convert them into an electrical signal; a digital phase modulation and demodulation module configured to perform a demodulation on the electrical signal according to the shifted frequency and each of the pseudo-random sequences, and obtain a phase difference between each of the sub-beams and the reference beam.

Abstract: Described herein are compositions of therapeutic extracellular vesicles, and methods and systems of producing the therapeutic extracellular vesicles. Also described herein are methods of treating a disease with the therapeutic extracellular vesicles.

Abstract: Therapeutic extracellular vesicles (EVs) containing high copies of functional nucleic acids and other biomolecules are produced in large quantities by laying donor cells on a surface of a chip, adding various plasmids, other transfection vectors and their combinations to a buffer on the chip, applying a pulsulatic electric field across the cells laid on top of the chip surface and plasmids/vectors buffer solution below the chip surface, and collecting the EVs secreted by the transfected cells. The chip surface has a three-dimensional (3D) nanochannel electroporation (NEP) biochip formed on it, capable of handling large quantities of the donor cells. The buffer is adapted for receiving plasmids and other transfection vectors.

Abstract: The embodiments of the present application disclose a method and an apparatus for determining flow rates of components of multiphase fluid. The method comprises: performing a first magnetization treatment and a second magnetization treatment on multiphase fluid in a pipeline in a target oil and gas well, respectively, to obtain first magnetized multiphase fluid and second magnetized multiphase fluid; determining a first echo train signal set and a second echo train signal set corresponding to the first magnetized multiphase fluid and the second magnetized multiphase fluid, respectively; determining contents of an oil phase component, a water phase component, and a gas phase component of the multiphase fluid at a specified horizon position, and determining a flow velocity of the multiphase fluid at the specified horizon position; and determining flow rates of the oil phase component, the water phase component and the gas phase component in the multiphase fluid.

Abstract: Devices for high throughput cell electroporation include a trapping component that at least partially defines an upper boundary of a microfluidic chamber. A cell trap array is patterned on the underside of the trapping component, and a channeling component is positioned beneath the trapping component. The channeling component includes a vertically oriented nanochannel array. The trapping component and the channeling component are positioned such that a given nanochannels is positioned beneath a cell trap. During use, fluid flow holds trapped cells in secure contact with the nanochannels beneath the cell trap. The device further includes upper and lower electrode layers for generating an electric field to electroporate trapped cells via the nanochannel array. A reservoir positioned beneath the channeling component can be filled transfection reagent solution. During electroporation, the transfection reagent solution travels through the nanochannel array during to transfect the trapped cells.

Abstract: The embodiments of the present application disclose a method and an apparatus for determining flow rates of components of multiphase fluid. The method comprises: performing a first magnetization treatment and a second magnetization treatment on multiphase fluid in a pipeline in a target oil and gas well, respectively, to obtain first magnetized multiphase fluid and second magnetized multiphase fluid; determining a first echo train signal set and a second echo train signal set corresponding to the first magnetized multiphase fluid and the second magnetized multiphase fluid, respectively; determining contents of an oil phase component, a water phase component, and a gas phase component of the multiphase fluid at a specified horizon position, and determining a flow velocity of the multiphase fluid at the specified horizon position; and determining flow rates of the oil phase component, the water phase component and the gas phase component in the multiphase fluid.

Abstract: Disclosed are peptides that contain up to about 35 amino acids, including a plurality of aromatic amino acid residues and either (i) an amino acid residue that is phosphorylated or sulfated, or (ii) an amino acid comprising an ester-moiety linked via peptide bond, or both (i) and (ii), wherein the peptide is capable of self-assembly to form nanofibrils in the presence of an enzyme that hydrolyzes the phosphate group, the sulfate group, or the ester-moiety. These peptides are enzymatically responsive hydrogelators, and they can be used to form pericellular hydrogels/nanofibrils upon exposure to target cells that secrete or express a surface bound ectoenzyme having hydrolase activity suitable to induce peptide gelation. These materials, and compositions containing the same, can be used for in vitro and in vivo cellular imaging, treating cancerous conditions, collecting a secretome from a cell upon which the pericellular hydrogels/nanofibrils form, and screening the collected secretome.