Patents by Inventor Junghoon Sohn

Junghoon Sohn has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 6838555
    Abstract: Disclosed are Hansenula polymorpha mutants useful as host cells through which various proteins can be produced as being intact at high yield and a process for preparing recombinant proteins using the host cells. Using various vectors, Hansenula polymorpha is made to be a mutant which is deprived of methanol assimilating ability and incapable of utilizing methanol as a carbon source. This Hansenula polymorpha mutant is used as a high yield host to produce recombinant proteins without continuous feeding of methanol, with the aid of an expression cassette carrying a promoter capable of inducing the expression at a low concentration of methanol. Further, the mutant is also lacking in carboxypeptidase Y, protease Y and/or carboxypeptidase a activity, so the recombinant protein of interest is not degraded at its carboxyl terminal when being expressed in the cell. Thus, intact recombinant protein can be obtained.
    Type: Grant
    Filed: November 14, 2001
    Date of Patent: January 4, 2005
    Assignee: Korea Research Institute of Bioscience & Biotechnology
    Inventors: Sangki Rhee, Euisung Choi, Hyunah Kang, Junghoon Sohn, Junghoon Bae, Moowoong Kim, Michael Agaphonov, Myungkuk Kim
  • Publication number: 20030108908
    Abstract: Disclosed are Hansenula polymorpha mutants useful as host cells through which various proteins can be produced as being intact at high yield and a process for preparing recombinant proteins using the host cells. Using various vectors, Hansenula polymorpha is made to be a mutant which is deprived of methanol assimilating ability and incapable of utilizing methanol as a carbon source. This Hansenula polymorpha mutant is used as a high yield host to produce recombinant proteins without continuous feeding of methanol, with the aid of an expression at a cassette carrying a promoter capable of inducing the expression at a low concentration of methanol. Further, the mutant is also lacking in carboxypeptidase Y, protease Y and/or carboxypeptidase &agr; activity, so the recombinant protein of interest is not degraded at its carboxyl terminal when being expressed in the cell. Thus, intact recombinant protein can be obtained.
    Type: Application
    Filed: July 26, 2002
    Publication date: June 12, 2003
    Inventors: Sangki Rhee, Euisung Choi, Hyunah Kang, Junghoon Sohn, Junghoon Bae, Moowoong Kim, Michael Agaphonov, Myungkuk Kim
  • Publication number: 20020150983
    Abstract: Disclosed are Hansenula polymorpha mutants useful as host cells through which various proteins can be produced as being intact at high yield and a process for preparing recombinant proteins using the host cells. Using various vectors, Hansenula polymorpha is made to be a mutant which is deprived of methanol assimilating ability and incapable of utilizing methanol as a carbon source. This Hansenula polymorpha mutant is used as a high yield host to produce recombinant proteins without continuous feeding of methanol, with the aid of an expression cassette carrying a promoter capable of inducing the expression at a low concentration of methanol. Further, the mutant is also lacking in carboxypeptidase Y, protease Y and/or carboxypeptidase a activity, so the recombinant protein of interest is not degraded at its carboxyl terminal when being expressed in the cell. Thus, intact recombinant protein can be obtained.
    Type: Application
    Filed: November 14, 2001
    Publication date: October 17, 2002
    Inventors: Sangki Rhee, Euisung Choi, Hyunah Kang, Junghoon Sohn, Junghoon Bae, Moowoong Kim, Michael Agaphonov, Myungkuk Kim