Abstract: Embodiments of the present disclosure provide composition and methods for recording an iterative nucleic acid editing event. The compositions and methods described herein comprise a first active target domain, comprising an editable recording sequence configured to hybridize with a first prime editing guide RNA (pegRNA) and one or more inactive truncated target domains comprising a non-editable sequence configured to not hybridize with the pegRNA, wherein the first pegRNA edits the first active target domain, wherein the pegRNA edit shifts the position of the recoding sequence from the editable sequence to the non-editable sequence, thereby changing the editable sequence to a non-editable sequence and the inactive truncated target domain to a second active target domain comprising a second recoding sequence configured to hybridize with a second pegRNA.

Abstract: The present application discloses a refractory material with the function of cleaning molten steel, a preparation method therefor and the use thereof. The material phase of the refractory material of the present application comprises one or more of CA6, CMA, corundum and ZrO2. The refractory material prepared by the present application has a high purity, good erosion resistance, good slag permeability resistance and high thermal shock stability, reduces the amount of refractory material eroded into molten steel, reduces the pollution of the molten steel, and can also give full play to the performance advantages of high-purity raw materials.

Abstract: The present invention belongs to the technical field of refractory materials, and disclosed are a high-purity compact calcium hexa-aluminate-based refractory material, a preparation method therefor, and a working lining using the same. The mixing ratio is adjusted according to the chemical composition of the final product to contain raw materials containing CaO, Al2O3 and ZrO2, the mixing ratio enabling the ratio of the chemical composition CaO:Al2O3:ZrO2 calculated according to parts by mass to be 45.5-95.5%:2.0-8.4%:0-50%; and the chemical composition are placed into a high-temperature furnace and a mold for hot-pressing is carried out, the maximum temperature is 1550-1800° C., and the hot-pressing strength is 0.5-30 MPa.

Abstract: Disclosed are a CA6-based thermally insulating refractory material with a medium volume density, a preparation method therefor, and the use thereof. The CA6-based thermally insulating refractory material with a medium volume density in the present invention has phases comprising CA6 and one or more selected from C2M2A14, C2M2A8, magnesium aluminate spinel, and corundum, and the refractory material has a high purity, good high temperature stability, a uniform structure, stable performance, a relatively low thermal conductivity, and good corrosion resistance to a metal, slag, etc.

Abstract: Disclosed in the present invention are a corrosion-resistant refractory material, preparation method therefor, and the use thereof. In the corrosion-resistant refractory material, a material phase of the refractory material comprises corundum and one or more material phases selected from CA6, C2M2A14, CM2A8, and ZrO2. The refractory material has low a low amount of a high-temperature liquid phase, a uniform pore structure, and good thermal shock stability; can be widely used in steel-making production lines and also in the refractory linings of rotary kilns, and has good erosion resistance and low thermal conductivity, and the performance thereof is obviously superior to that of many existing refractory materials such as silico carbide-mullite bricks and magnesia-alumina spinel bricks.

Abstract: An innovative equipment system comprises a tap water reservoir system, a disinfectant reactor system, a pump system, a disinfection and safety door spraying system, a sewage recycling system and a sewage treatment system, wherein the tap water reservoir system is mainly configured to store tap water in advance according to actual cargo volumes and disinfection requirements, and generally in tons; and the disinfectant reactor system comprises boron-doped diamond electrode modules, a reactor indicating lamp module, a visible reactor, a smart control screen, at least one water tank and a structure protection module. The Innovative disinfection and sterilization equipment system can easily kill bacteria and viruses such as Escherichia coli, Staphylococcus aureus, Salmonella typhimurium, and new coronavirus in a short time and enhance disinfection efficiency.

Abstract: The present invention provides an optimization control method for an aero-engine transient state based on reinforcement learning, and belongs to the technical field of aero-engine transient states. The method comprises: adjusting an existing twin-spool turbo-fan engine model as a model for invoking a reinforcement learning algorithm; to simultaneously satisfy high level state space and continuous action output of a real-time model, designing an Actor-Critic network model; designing a deep deterministic policy gradient (DDPG) algorithm based on an Actor-Critic frame, to simultaneously solve the problems of high-dimensional state space and continuous action output; training the model after combining the Actor-Critic frame with the DDPG algorithm; and obtaining the control law of engine acceleration transition from the above training process, and using the method to control an engine acceleration process.

Abstract: A fluid separating device includes a cylinder; a plurality of separators disposed around the cylinder; a first elastic piece disposed between one of the separators and the cylinder and applying an elastic force to the separator outward in a radial direction of the cylinder; a mandrel passing through the cylinder axially and configured to reciprocate between an expanded position and a contracted position in an axial direction of the cylinder; an elastic energy storage device slidably passing through the cylinder along the radial direction of the cylinder, the elastic energy storage device having one end connected to the mandrel and another end connected to the separator, the elastic energy storage device being configured to apply another elastic force to the mandrel in a direction from the contracted position to the expanded position; a first locking structure disposed on the cylinder; and a second locking structure disposed on the mandrel.

Abstract: Provided is a method for preparing copper azide and cuprous azide encapsulated by conductive metal-organic framework. The method uses a conductive copper-containing metal-organic framework material as a precursor, and completes the azidation of the precursor by means of a liquid-solid electrochemical azidation reaction. Copper azide and cuprous azide nanocrystals are highly uniformly embedded within a conductive framework, which may effectively avoid the agglomeration of copper azide and cuprous azide, and reduce static charge generated by friction, displacement, and the like. Meanwhile, the conductive framework may promote the effective transfer of charge, avoid the accumulation of static charge, and improve the electrostatic safety.

Abstract: A water purification system comprising a thiol-functionalized graphene oxide/activated carbon composite material, a filter and one or more capacitive deionization cells with applications in removal of water contaminants such as heavy metal ions without generation of wastewater.

Abstract: A manufacturing method of a low heat-conducting magnesium-aluminium spinel brick includes: (1) evenly mixing sintered magnesia, fused magnesia, magnesium-aluminium spinel and corundum to prepare flame retardant coating raw material mixed powder, adding naphthalene binder to the flame retardant coating raw material mixed powder to prepare the flame retardant coating raw materials after evenly mixing; (2) evenly mixing forsterite, fayalite and magnesia, adding the naphthalene binder to the mixed powder, moulding, drying, and then burning to obtain aggregate composite hortonolite raw materials; adding the naphthalene binder to the aggregate composite hortonolite having granularity ?5 mm to prepare the thermal insulating layer raw materials after evenly mixing; (3) spacing and loading the flame retardant coating raw materials and the thermal insulating layer raw materials in a mold, pressing into green bricks, keeping the green bricks at a temperature of 110° C.

Abstract: Provided is a vehicle generator control method. An engine ECU controls the generator according to an operating condition of the engine, and the control process is as follows: when the engine ECU determines that the engine is in a starting condition, the engine ECU controls a generator separately excited loop to be disconnected, so that the generator does not generate electricity; and when the engine ECU determines that the engine enters an idling condition, the engine ECU controls the generator separately excited loop to be closed, so that the generator is started to generate electricity, and after the generator is started to generate electricity, the generator carries out normal electricity generation until the engine stops operating. Through the vehicle generator control method, the starting load of the engine can be effectively reduced, and cold starting capacity is improved. Also provided is a vehicle generator control system.

Abstract: A manufacturing method of a low heat-conducting magnesium-aluminium spinel brick includes: (1) evenly mixing sintered magnesia, fused magnesia, magnesium-aluminium spinel and corundum to prepare flame retardant coating raw material mixed powder, adding naphthalene binder to the flame retardant coating raw material mixed powder to prepare the flame retardant coating raw materials after evenly mixing; (2) evenly mixing forsterite, fayalite and magnesia, adding the naphthalene binder to the mixed powder, moulding, drying, and then burning to obtain aggregate composite hortonolite raw materials; adding the naphthalene binder to the aggregate composite hortonolite having granularity ?5 mm to prepare the thermal insulating layer raw materials after evenly mixing; (3) spacing and loading the flame retardant coating raw materials and the thermal insulating layer raw materials in a mold, pressing into green bricks, keeping the green bricks at a temperature of 110° C.

Abstract: Systems and methods for detecting ions in samples. In one embodiment, the system includes a field-effect transistor sensor and an electronic controller. The field-effect transistor sensor is in contact with the sample and includes a first electrode and a second electrode. The electronic controller is coupled to the field-effect transistor sensor. The electronic controller is configured to apply a pulse wave excitation signal to the first electrode. The electronic controller is also configured to receive a response signal from the second electrode. The electronic controller is further configured to determine an electrical characteristic of the field-effect transistor sensor based on the response signal. The electronic controller is also configured to determine an amount of the ions in the sample based on the electrical characteristic of the field-effect transistor sensor.

Abstract: A rapid activation method of an electrically controlled common ail engine. The method includes: arranging, between an oil outlet of a fuel transfer pump and an oil inlet of a fuel injection pump in the electrically controlled common rail engine, a gas discharging device; and communicating an outlet of the gas discharging device with a fuel tank via a pipe to discharge a gas present in fuel during a normal system operation. By arranging the gas discharging device at an oil feeding port of a fine filter, the method can be utilized to discharge, via the gas discharging device to the outside, air in the fine filter, thereby implementing active gas discharging, and effectively increasing activation performance of the engine.

Abstract: Provided is a vehicle generator control method. An engine ECU controls the generator according to an operating condition of the engine, and the control process is as follows: when the engine ECU determines that the engine is in a starting condition, the engine ECU controls a generator separately excited loop to be disconnected, so that the generator does not generate electricity; and when the engine ECU determines that the engine enters an idling condition, the engine ECU controls the generator separately excited loop to be closed, so that the generator is started to generate electricity, and after the generator is started to generate electricity, the generator carries out normal electricity generation until the engine stops operating. Through the vehicle generator control method, the starting load of the engine can be effectively reduced, and cold starting capacity is improved. Also provided is a vehicle generator control system.

Abstract: A care-free activation system of an electrically controlled common rail engine. The care-free activation system includes a fuel tank, a fuel transfer pump and a fine filter. A gas discharging device discharging fuel gas is arranged between the fuel transfer pump and the fine filter. An outlet of the gas discharging device communicates with the fuel tank via an oil pipe. In the care-free activation system of the electrically controlled common rail engine, by arranging the gas discharging device at an oil feeding port of a fine filter, air in the fine filter can be discharged via the gas discharging device, thereby implementing active gas discharging, and effectively increasing activation performance of the engine.

Abstract: The present disclosure relates generally to the field of lithium-ion batteries and battery modules. More specifically, the present disclosure relates to Si-based anode materials for use as anode active materials for lithium-ion batteries. One example includes micron/nano-scale structures that include a carbonate template structure and a silicon (Si) layer conformally deposited over the carbonate template. Another example includes a hollow, micron/nano-scale silicon structure having an oxygen content less than approximately 9%, wherein the interior of the hollow micron/nano-scale silicon structure is substantially free of carbon.

Abstract: Provided herein is a field-effect transistor based sensor for real-time detection of water contaminants and methods of use thereof.

Abstract: Compositions that include liquid metal particles and a carbon-based scaffold are disclosed. The composition may be used in a number of different applications, including battery and capacitor applications. Also disclosed are methods of making liquid metal-based compositions.