Abstract: The present invention is to provide an immunochromatography method which is capable of performing a detection with high-sensitivity or reduced false-positives by suppressing the occurrence of false-positive when a signal is amplified. An immunochromatography method includes in a state of where a complex of a test substance and a labeling substance containing a metal coupled with a first binding substance for the test substance is formed, developing the complex on an insoluble carrier in presence of a protease hydrolyzate of protein; capturing the test substance and the labeling substance at a detection site of the insoluble carrier containing a material which has a binding property to the first binding substance for the test substance or a second binding substance for the test substance; and detecting the test substance by amplifying the labeling substance captured.

Abstract: A plurality of kinds of liquids, which are of at least three kinds, containing (a) a test body solution containing at least one kind of an analyte, and (b) at least two kinds of liquids selected from the group consisting of a reagent solution, an amplifying solution, and a detecting solution, are fed to a detection site containing a specific binding substance with respect to the analyte. A qualitative analysis or a quantitative analysis of the analyte contained in the test body solution is thereby performed. Directions of liquid feeding of all of the plurality of the kinds of the liquids vary from one another, and the plurality of the kinds of the liquids are caused to intersect with one another at the detection site.

Abstract: An immobilization method for immobilizing a physiologically active substance on a solid phase carrier, the method including: bringing the solid phase carrier into contact with an acid anhydride functional group-containing silane coupling agent represented by the following Formula (I); and carrying out a process of binding of the physiologically active substance to the acid anhydride functional group while maintaining the solid phase carrier after the contact at a temperature within the range of 0° C. to 60° C.; a physiologically active substance-immobilized carrier, and a carrier for immobilization are provided. Further, a carrier including a porous material treated with an acid anhydride functional group-containing silane coupling agent represented by the following Formula (I), a blocking agent that is immobilized to the porous material; and a method for producing it is provided.

Abstract: A chromatographic measurement apparatus for measuring a color development state of an insoluble carrier with a sample solution and a label solution developed thereon to test a test article, the insoluble carrier including a test detection area where a material that binds specifically to a test article is immobilized and a control detection area used for determining an end of measurement. The chromatographic measurement apparatus includes: a determining unit for carrying out determination of validity and determination of necessity of amplification of a test result of the test article; and an amplifying unit for amplifying the color development state according to the result of the determination of necessity of amplification by the determining unit. The amplification is carried out only when it is determined that amplification is necessary.

Abstract: Provided are a base plate and a method for thyroxine immunoassay, which are capable of decreasing the signal change rate caused by temperature change in a high-sensitivity immunoassay method for thyroxine. A base plate for thyroxine immunoassay which has a conjugate of thyroxine or a derivative thereof and albumin immobilized thereon, and in which the ratio of the molecule number of thyroxine or a derivative thereof to the molecule number of albumin in the immobilized conjugate, is disclosed.

Abstract: An immobilization method for immobilizing a physiologically active substance on a solid phase carrier, the method including: bringing the solid phase carrier into contact with an acid anhydride functional group-containing silane coupling agent represented by the following Formula (I); and carrying out a process of binding of the physiologically active substance to the acid anhydride functional group while maintaining the solid phase carrier after the contact at a temperature within the range of 0° C. to 60° C.; a physiologically active substance-immobilized carrier, and a carrier for immobilization are provided. Further, a carrier including a porous material treated with an acid anhydride functional group-containing silane coupling agent represented by the following Formula (I), a blocking agent that is immobilized to the porous material; and a method for producing it is provided.

Abstract: The present invention provides an immunochromatographic device, which contains the following (a) and (b): (a) a first device part holding a first insoluble carrier used for developing a complex formed with an analyte and a labeling substance comprising a metal labeled with a first binding substance that can bind to the analyte and capturing the analyte and the labeling substance at a reaction portion containing a second binding substance that can bind to the analyte, and (b) a second device part holding a second insoluble carrier used for developing a liquid and a third insoluble carrier used for absorbing a liquid, in such a way that the first insoluble carrier does not come into contact with the second insoluble carrier and the third insoluble carrier.

Abstract: The present invention is to provide an immunochromatography method which is capable of performing a detection with high-sensitivity or reduced false-positives by suppressing the occurrence of false-positive when a signal is amplified. An immunochromatography method includes in a state of forming a complex from a test substance and a labeling substance containing a metal modified with a first binding substance for the test substance, developing the complex on an insoluble carrier in presence of a protease hydrolyzate of protein; capturing the test substance and the labeling substance in a detection site on the insoluble carrier containing a substance which has a binding property to a second binding substance for the test substance or the first binding substance for the test substance; and detecting the test substance by amplifying the labeling substance captured.

Abstract: Provided is an immunochromatography method that enables highly sensitive detection by reducing the background density of a non-measurement site in the immunochromatography method. The immunochromatography method includes developing a complex of a substance to be tested and a labeling substance containing a metal modified with a first binding substance for the substance to be tested on an insoluble carrier in the presence of a surfactant having a steroid skeleton while the substance to be tested and the labeling substance form the complex; and detecting the complex of the substance to be tested by capturing the substance to be tested and the labeling substance on a detection site of the insoluble carrier containing a second binding substance for the substance to be tested or a substance that can bind to the first binding substance for the substance to be tested.

Abstract: A device for assay can evenly develop solution, and performs highly accurate and sensitive measurement. A first device part (10) maintains a second insoluble carrier (12) and a third insoluble carrier (13) in such a manner that they overlap with each other at a detection portion (14) of a first insoluble carrier (11). These three carriers (11), (12) and (13) are housed not in contact with each other. A pressing unit (18) having a pressing surface (18a) that is parallel to the detection portion (14) is provided on an inner surface of the second device part (20) facing the detection portion (14). The pressing surface (18a) is displaced by being pressed toward the detection portion (14), and presses, from the upper side of the first insoluble carrier (11), the second insoluble carrier (12) and the third insoluble carrier (13) onto the first insoluble carrier (11). The first device part (10) and the second device part (20) are joined together.

Abstract: It is an object of the present invention to provide a measurement kit for developing a first developing solution and a second developing solution from different directions to suppress background noise, and an immunochromatography kit. The present invention provides a measurement kit, which comprises a first developing member for supplying a first developing solution and a second developing member for supplying a second developing solution, wherein the developing direction of the first developing solution is allowed to intersect with the developing direction of the second developing solution, so that development is carried out by developing the first and second developing solutions in different developing directions, and a water absorbent portion is established on the downstream of each of the developing directions.

Abstract: A object of the present invention is to provide an immunochromatography method that makes it possible to rapidly detect an ultratrace amount of an analyte that has been impossible to analyze by conventional immunochromatography methods. The present invention provides an immunochromatography method, which comprises developing an analyte and a labeling substance which is modified with a first binding substance against the analyte in a mixed state on a porous carrier and capturing the analyte and the label at a reaction site on the porous carrier having a second binding substance against the analyte or a substance capable of binding to the first binding substance against the analyte, so as to detect the analyte, wherein the labeling substance having an average particle size of 1 ?m or more and 20 ?m or less is detected.

Abstract: It is an object of the present invention to provide a measurement kit for developing a first developing solution and a second developing solution from different directions to suppress background noise, and an immunochromatography kit. The present invention provides a measurement kit, which comprises a first developing member for supplying a first developing solution and a second developing member for supplying a second developing solution, wherein the developing direction of the first developing solution is allowed to intersect with the developing direction of the second developing solution, so that development is carried out by developing the first and second developing solutions in different developing directions, and a water absorbent portion is established on the downstream of the developing directions.

Abstract: It is an object of the present invention to provide an immunochromatography method capable of measuring a sample in a low concentration range and a high concentration range by setting the measurable range of the sample much wider than the conventional measurable range.

Abstract: A chromatographic measurement apparatus for measuring a color development state of an insoluble carrier with a sample solution and a label solution developed thereon to test a test article, the insoluble carrier including a test detection area where a material that binds specifically to a test article is immobilized and a control detection area used for determining an end of measurement. The chromatographic measurement apparatus includes: a determining unit for carrying out determination of validity and determination of necessity of amplification of a test result of the test article; and an amplifying unit for amplifying the color development state according to the result of the determination of necessity of amplification by the determining unit. The amplification is carried out only when it is determined that amplification is necessary.

Abstract: It is an object of the present invention to provide a method for simply mixing two or more types of liquids in a porous carrier.

Abstract: An immobilization method for immobilizing a physiologically active substance on a solid phase carrier, the method including: bringing the solid phase carrier into contact with an acid anhydride functional group-containing silane coupling agent represented by the following Formula (I); and carrying out a process of binding of the physiologically active substance to the acid anhydride functional group while maintaining the solid phase carrier after the contact at a temperature within the range of 0° C. to 60° C.; a physiologically active substance-immobilized carrier, and a carrier for immobilization are provided. Further, a carrier including a porous material treated with an acid anhydride functional group-containing silane coupling agent represented by the following Formula (I), a blocking agent that is immobilized to the porous material; and a method for producing it is provided.

Abstract: The present invention provides an immunochromatographic device, which contains the following (a) and (b): (a) a first device part holding a first insoluble carrier used for developing a complex formed with an analyte and a labeling substance comprising a metal labeled with a first binding substance that can bind to the analyte and capturing the analyte and the labeling substance at a reaction portion containing a second binding substance that can bind to the analyte, and (b) a second device part holding a second insoluble carrier used for developing a liquid and a third insoluble carrier used for absorbing a liquid, in such a way that the first insoluble carrier does not come into contact with the second insoluble carrier and the third insoluble carrier.

Abstract: It is an object of the present invention to provide a blotting detection method capable of quick detection of a very tiny amount of an analysis target. The present invention provides a blotting detection method which comprises moving an analyte held on a first carrier with a developing solution, and adsorbing the analyte onto a second carrier, wherein the analyte is labeled with metal fine particle and is detected by sensitization with use of a silver-containing compound and a reducing agent for silver ion, and the labeling substance having a size of not less than 1 ?m and not more than 20 ?m in the average particle size at the time of detection is detected.

Abstract: It is an object of the present invention to provide a measurement kit for developing a first developing solution and a second developing solution from different directions to suppress background noise, and an immunochromatography kit. The present invention provides a measurement kit, which comprises a first developing member for supplying a first developing solution and a second developing member for supplying a second developing solution, wherein the developing direction of the first developing solution is allowed to intersect with the developing direction of the second developing solution, so that development is carried out by developing the first and second developing solutions in different developing directions, and a water absorbent portion is established on the downstream of the developing directions.