Abstract: A CRISPR-Cas3 system was successfully established in a eukaryotic cell.

Abstract: A CRISPR-Cas3 system was successfully established in a eukaryotic cell.

Abstract: A CRISPR-Cas3 system was successfully established in a eukaryotic cell.

Abstract: The purpose of the present invention is to provide a technique by which a site-specific homozygote can be efficiently acquired by increasing cross efficiency and a homozygote in a genome modification-free state can be easily obtained. A method for producing homozygous cells, said method including: (A) a step for, into cells having a heterozygous mutation at a target site, introducing heterozygouse allele specific cleavage of DNA double strand of homologous chromosomes and thus inducing crossing in the presence of a Bloom's syndrome protein inhibitor to thereby give homozygous cells at the target site; and (B) a step for selecting the homozygous cells.

Abstract: A CRISPR-Cas3 system was successfully established in a eukaryotic cell.

Abstract: This is intended to provide a technique for providing a stem cell having a mutation in both alleles (a pair of alleles). A method for producing a stem cell having a mutation in both chains of alleles which comprises: A) the step of providing a stem cell; B) the step of preventing Blm alleles from functioning in the stem cell; and C) the step of inducing mutation in the stem cell. It is also intended to provide a library of stem cells having a mutation in both chains of alleles wherein stem cells involved in the library have the mutation transferred thereinto over the entire genome.

Abstract: To comprehensively modify genome, it is intended to develop a transposition system of the copy and paste type which has an improved efficiency. This object has been achieved by the finding that an LTR retrotransposon is partly usable in a transposition system. Namely, a technique of efficiently transferring a foreign gene into a cell by using a transposon. More specifically speaking, a complete IPA element and a functional promoter sequence are found out. It is clarified that, without a combination of them, a retrotransposon cannot exert its function.

Abstract: A technique for efficiently introducing a foreign gene into cells with the use of transposons. In particular, a technique for efficiently preparing a transgenic organism with the use of a transposon having its transposition activity strikingly enhanced through methylation of a sequence containing the transposon. The methylation is retained even after incorporation in a genome, and now can be utilized in actual gene incorporation in a genome. This technique can realize strikingly efficient gene transformation as compared with the a method of preparing a transgenic organism with the use of conventional transposons.

Abstract: A gene-mutated animal such as a mouse which comprises a mutant prsenilin-1 gene comprising a DNA having a sequence encoding a mutant presenilin-1 protein in which an amino acid is substituted with a different amino acid in an amino acid sequence of a presenilin-1 protein; for example, a mutant presenilin protein in which isoleucine at position 213 is substituted with an amino acid other than isoleucine, e.g., threonine, in a mouse presenilin-1 protein. The animal is useful as an animal model which has pathological conditions closer to a human patient with Alzheimer's disease.

Abstract: The present invention provides a transgenic non-human mammal containing a nonself-contained transposon and/or a transposase gene and a method of producing the same, an animal model of disease, and a method of clarifying gene function.

Abstract: A light transmission module is disclosed, having a light receiving element module, in which a photodiode and a coupling portion between the photodiode and a first optical fiber are hermetically sealed, and a light emitting element module, in which a laser diode driven by an electric signal and a coupling portion between said laser diode and a second optical fiber are also hermetically sealed. A two-sided print board is provided, on the two sides of which electric circuits for a transmitting system and a receiving system are mounted. The electric circuits for the transmitting and receiving circuits are; isolated electrically from each other, and are sealed in one body by molds, and accommodated in cases, together with the light receiving element module and the light emitting element module.