Abstract: Desired components can be simply and economically separated and recovered from a sample solution having a complicated composition through a liquid chromatography by passing the sample solution through a separation column, thereby separating components contained therein in the separation column, then selectively passing the eluate from the separation column through a trapping column according to a desired component, thereby trapping the desired component in the trapping column, while discarding or recycling the effluent eluate from the trapping column, and then passing another eluting solution composed of a volatile substance when dried under reduced pressure through the trapping column, thereby recovering eluate containing the desired component from the trapping column.

Abstract: A disease diagnostic method and apparatus in which a two-dimensional pattern diagram representing the relation between integrated values of peaks and the retention times in a chromatogram of substances in a body fluid of a subject is obtained to be geographically compared, for disease diagnostic purpose, with a two-dimensional pattern diagram representing the relation between integrated values of peaks and the retention times in a chromatogram obtained through separating and detecting substances in a body fluid of a normal person, and also with two-dimensional pattern diagrams representing the relation between integrated values of peaks and the retention times in chromatograms obtained through separating and detecting substances in a body fluid of abnormal patients classified by diseases.