Abstract: The present invention is directed to provide new PCR measuring method and device. As one embodiment of the present invention, a DNA detection method for detecting DNA in a droplet being present in oil, the droplet containing the DNA and a fluorescent labeled probe, the fluorescent labeled probe being hybridized to the DNA, the method including: a first step of amplifying the DNA in the droplet by a nucleic acid amplification reaction; and a second step of measuring a melting temperature of the fluorescent labeled probe and the DNA in the droplet is provided.

Abstract: The present invention is directed to provide new PCR measuring method and device. As one embodiment of the present invention, a DNA detection method for detecting DNA in a droplet being present in oil, the droplet containing the DNA and a fluorescent labeled probe, the fluorescent labeled probe being hybridized to the DNA, the method including: a first step of amplifying the DNA in the droplet by a nucleic acid amplification reaction; and a second step of measuring a melting temperature of the fluorescent labeled probe and the DNA in the droplet is provided.

Abstract: Provided are a method and a system for accurately distinguishing a micropartition in which a target gene or target DNA is placed and a micropartition in which a pseudogene is placed by a measuring device and accurately counting the target gene or target DNA in digital PCR using melting curve analysis. The method includes the steps of: placing a DNA solution in each of a plurality of partitions; performing PCR; changing a temperature of each of the partitions and measuring a fluorescence intensity; calculating a melting temperature of a double strand DNA for each of the partitions; counting the number of partitions for each type of the DNA; outputting the number of partitions counted for each type of the DNA; and discriminating the first gene and the pseudogene based on the melting temperature and the number of the counted partitions.

Abstract: The purpose of the present invention is to provide a novel digital PCR analysis method. In the digital PCR analysis method disclosed herein, a method for detecting DNA is used, which includes the steps of: dividing a DNA solution containing a fluorescent-labeled probe or a DNA intercalator and a plurality of DNAs to be detected into a plurality of compartments; carrying out PCR in the compartments; measuring a fluorescence intensity in association with a change in temperature; calculating a melting temperature from a melting curve for a DNA double strand measured on the basis of a change in fluorescence intensity, which is associated with the change in temperature; and calculating a temperature difference between two points with a slope of a predetermined value on a melting curve indicating a change in the fluorescence intensity.

Abstract: A heating apparatus (2) includes a high frequency power supply (3), a left conductive plate (4), a right conductive plate (5), a left heating coil (11), and a right heating coil (12). The left heating coil (11) includes a left conductor portion (21). The left conductor portion (21) faces a gear tooth (7a) of a helical gear (7) and extends in a direction orthogonal to the direction in which the gear tooth (7a) extends. The left heating coil (11) includes a focusing magnetic body (31) which focuses magnetic flux in the left conductor portion (21) and concentrates the magnetic flux on a surface of the gear tooth (7a). The left heating coil (11) includes an upper inducing magnetic body (32) which induces a part of the magnetic flux flowing in a tooth root of the gear tooth (7a) into a tooth tip of the gear tooth (7a).

Abstract: The present disclosure provides a technique capable of manufacturing a measurement cell having a high specimen utilization ratio in the case of using a measurement cell that introduces a specimen into a surface hole. In the measurement cell manufacturing method according to the present disclosure, a measurement cell includes a channel wall protruding from the lower surface substrate toward the through-hole, and a specimen solution is introduced into a lower surface side space to introduce the specimen into the through-hole (see FIG. 1).

Abstract: A digital PCR measuring apparatus capable of measuring a melting curve with high accuracy is provided. The digital PCR measuring apparatus includes a temperature adjuster that controls a temperature of a sample container including a plurality of minute regions, a fluorescence measurement part that measures fluorescence intensity of a plurality of minute regions, and a controller that controls the temperature adjuster and the fluorescence measurement part. The controller controls the temperature adjuster to raise a temperature of the sample container, and, after removing air bubbles generated in the sample container, measures fluorescence intensity of a plurality of minute regions while controlling the temperature adjuster to lower the temperature of the sample container, and measures a melting curve of a plurality of the minute regions.

Abstract: The present invention addresses the problem of providing a novel digital PCR analysis method. One embodiment of the novel digital PCR analysis method is a DNA detection method including the steps of: partitioning a DNA solution containing a fluorescent-labeled probe or a DNA intercalator and a target DNA to be detected into a plurality of compartments; carrying out a nucleic acid amplification reaction in the compartments; measuring a fluorescence intensity in association with a temperature change; calculating a melting temperature of a DNA double strand based on a change in the fluorescence intensity in association with the temperature change; and calculating a ratio of a fluorescence intensity at a second temperature that is lower than a first temperature in association with the temperature change to that at the first temperature.

Abstract: The present invention is directed to provide new PCR measuring method and device. As one embodiment of the present invention, a DNA detection method for detecting DNA in a droplet being present in oil, the droplet containing the DNA and a fluorescent labeled probe, the fluorescent labeled probe being hybridized to the DNA, the method including: a first step of amplifying the DNA in the the droplet by a nucleic acid amplification reaction; and a second step of measuring a melting temperature of the fluorescent labeled probe and the DNA in the droplet is provided.

Abstract: An organic electroluminescence (EL) element including an anode, a light-emitting layer above the anode, a first functional layer on and in contact with the light-emitting layer, a second functional layer on and in contact with the first functional layer, and a cathode above the second functional layer. A lowest unoccupied molecular orbital (LUMO) level of the first functional layer is lower than at least one of a LUMO level of the second functional layer and a Fermi level of a metal material included in the second functional layer.

Abstract: An organic electroluminescence (EL) element including an anode, a cathode opposing the anode, a light-emitting layer between the anode and the cathode, a hole transport layer in contact with the light-emitting layer, between the light-emitting layer and the anode, and an electron transport layer in contact with the light-emitting layer, between the light-emitting layer and the cathode. A difference between a lowest unoccupied molecular orbital (LUMO) level of an organic material included in the light-emitting layer and a LUMO level of an organic material included in the electron transport layer is greater than a difference between a highest occupied molecular orbital (HOMO) level of an organic material included in the hole transport layer and a HOMO level of the organic material included in the light-emitting layer.

Abstract: A heating apparatus (2) includes a high frequency power supply (3), a left conductive plate (4), a right conductive plate (5), a left heating coil (11), and a right heating coil (12). The left heating coil (11) includes a left conductor portion (21). The left conductor portion (21) faces a gear tooth (7a) of a helical gear (7) and extends in a direction orthogonal to the direction in which the gear tooth (7a) extends. The left heating coil (11) includes a focusing magnetic body (31) which focuses magnetic flux in the left conductor portion (21) and concentrates the magnetic flux on a surface of the gear tooth (7a). The left heating coil (11) includes an upper inducing magnetic body (32) which induces a part of the magnetic flux flowing in a tooth root of the gear tooth (7a) into a tooth tip of the gear tooth (7a).

Abstract: The present invention was made to provide novel methods for detecting DNA and devices therefor. Specifically, the present invention is a method of detecting DNA including the steps of: adding, to an oil, a fluorescently-labeled probe, a DNA intercalator, and a DNA solution containing a target DNA to produce droplets; performing PCR on the droplets; and measuring fluorescence from the fluorescently-labeled probe and fluorescence from the DNA intercalator, wherein the DNA solution has a concentration at which each droplet is produced so as to contain one or less target DNA molecule. In addition, the present invention is a DNA detection device including a droplet production unit, a thermal cycler unit, and a fluorescence detection unit which perform these steps.

Abstract: An organic electroluminescence (EL) element including an anode, a light-emitting layer above the anode, a first functional layer on and in contact with the light-emitting layer, a second functional layer on and in contact with the first functional layer, and a cathode above the second functional layer. A lowest unoccupied molecular orbital (LUMO) level of the first functional layer is lower than at least one of a LUMO level of the second functional layer and a Fermi level of a metal material included in the second functional layer.

Abstract: An organic electroluminescence (EL) element including an anode, a cathode opposing the anode, a light-emitting layer between the anode and the cathode, a hole transport layer in contact with the light-emitting layer, between the light-emitting layer and the anode, and an electron transport layer in contact with the light-emitting layer, between the light-emitting layer and the cathode. A difference between a lowest unoccupied molecular orbital (LUMO) level of an organic material included in the light-emitting layer and a LUMO level of an organic material included in the electron transport layer is greater than a difference between a highest occupied molecular orbital (HOMO) level of an organic material included in the hole transport layer and a HOMO level of the organic material included in the light-emitting layer.

Abstract: A vibration damping device includes: a cylindrical first attachment member and a second attachment member; and an annular first elastic body and an annular second elastic body, wherein, a liquid chamber is formed between the elastic bodies, elastic division walls that divide the liquid chamber into a plurality of pressure receiving liquid chambers are arranged, wherein the elastic division wall is joined to the first elastic body, and is also in abutment with the second elastic body, wherein the second attachment member is divided in the axial direction into: a first divided body to which an inner peripheral edge portion of the first elastic body is joined; and a second divided body to which an inner peripheral edge portion of the second elastic body is joined, and wherein the inner peripheral edge portions of the elastic bodies are located side by side in the axial O direction.

Abstract: Provided is an impression tray which enables impressions of upper and lower jaws to be taken at the same time, and on which an impression material can be heaped sufficiently. The impression tray includes an outer frame that curves and extends from an incisors side toward a molars side, an inner frame that extends with a distance away from the outer frame, a connecting part that one end of which is fixed to the outer frame and another end of which is fixed to the inner frame, a face material that is spread over the distance between the outer frame and the inner frame, and a handle that is arranged at the incisors side of the outer frame. A portion of the outer frame at the incisors side is highest position, and its height is for no less than 2 mm and no more than 15 mm.

Abstract: A vibration damping device includes: a cylindrical first attachment member and a second attachment member; and an annular first elastic body and an annular second elastic body, wherein, a liquid chamber is formed between the elastic bodies, elastic division walls that divide the liquid chamber into a plurality of pressure receiving liquid chambers are arranged, wherein the elastic division wall is joined to the first elastic body, and is also in abutment with the second elastic body, wherein the second attachment member is divided in the axial direction into: a first divided body to which an inner peripheral edge portion of the first elastic body is joined; and a second divided body to which an inner peripheral edge portion of the second elastic body is joined, and wherein the inner peripheral edge portions of the elastic bodies are located side by side in the axial O direction.

Abstract: A solution monitoring system is provided for gathering, filtering, and analyzing information associated with solutions for managing monitoring, upgrades, and migrations. Telemetry may be recorded for various types of solution events and various properties of solutions as part of an add-in intelligence. Furthermore, add-in management tools are provided for administrators and end users.