Abstract: An alignment method for screen printing process includes: attaching a transparent film on a to-be-printed substrate in such a manner that the transparent film covers at least a to-be-printed region of the to-be-printed substrate, forming a printed pattern including an alignment mark on the transparent film by using a screen printing plate to imprint printed material onto the transparent film, and comparing whether the alignment mark on the transparent film is aligned with an alignment mark on the to-be-printed substrate. When the alignment mark on the transparent film is aligned with the alignment mark on the to-be-printed substrate, it is determined that current positions of the screen printing plate and the to-be-printed substrate are final alignment positions. When the alignment mark on the transparent film is not aligned with the alignment mark on the to-be-printed substrate, the current position of the screen printing plate or the to-be-printed substrate is adjusted.