Abstract: The present invention belongs to the technical field of microorganisms. Disclosed is a strain of lactic-acid-stress-tolerant Zygosaccharomyces bailii capable of achieving high-yield production of ethanol and flavor compounds. The Zygosaccharomyces bailii CCTCC NO: M 2023729 of the present invention is screened from Chinese Maotai-flavor liquor yeast, and the strain is not only tolerant of lactic acid stress, but also has lactic acid degrading capability, and can also achieve high-yield production of ethanol and various flavor compounds under the stress of lactic acid. The strain of the present invention can be prepared into a microbial agent for use in the fermentation production process of Maotai-flavor liquor, and can significantly increase the content of ethanol and flavor compounds in fermented grain.