Abstract: The present invention discloses a method and a device for non-destructive sorting of fertilization information of hatching eggs before incubation. The method comprises the following steps: obtaining a first time sequence feature of hatching eggs and collecting spectrum data of the hatching eggs according to the first time sequence feature; preprocessing the spectrum data; selecting the preprocessed spectrum data to obtain a feature band; constructing a hatching egg classification neural network model and training the hatching egg classification neural network model; classifying the hatching egg information according to the feature band by using the trained hatching egg classification neural network model to obtain fertilization information; and obtaining a second time sequence feature of the hatching eggs, and sorting the hatching eggs. The present invention improves the precision of detecting the fertilization information of hatching eggs.