Abstract: The present invention provides a mRNA composition for microneedle administration and its application. The composition includes an aqueous phase solution and a lipid solution, wherein the lipid solution encapsulates the substance in the aqueous phase solution to form lipid nanoparticles (LNPs), and the aqueous phase solution includes mRNA encoding the corresponding protein and a buffer. It was found that an improved buffer pH could substantially increase the drug loading capacity of LNPs, and reduce the use level of composition and excipients and the dose for microneedle administration with less toxic side effects and better therapeutic effects, and the microneedle administration technology could achieve increased effective expression, decreased expression in the liver, and prolonged in vivo expression of mRNA composition, and increased antibody titer in response to low-dose mRNA in vivo. The present invention really realizes microneedle intradermal administration of mRNA composition.

Abstract: In some aspects, methods for controlling a plasma arc in a plasma torch of a plasma cutting system in a low operating current mode can include: receiving, by a computing device within the plasma power supply, a command to begin a plasma processing operation; generating a pilot arc command to generate a pilot arc within the plasma torch, the generating of the pilot arc command including directing an electrical signal and a gas flow to the plasma torch, the electrical signal being configured to generate the pilot arc at a current having a first arc amperage magnitude; and generating an operational arc command to facilitate a transition from the pilot arc to an operational plasma arc, the generating of the operational arc command including adjusting the current directed to the plasma torch to be a second arc amperage magnitude that is lower than the first arc amperage magnitude.

Abstract: In some aspects, methods of disengaging a plasma control circuit within a plasma arc torch of a plasma processing system to place the torch into a safety-locked mode can include providing: a plasma control circuit configured to convey a current from the plasma cutting system through the plasma arc torch to one or more consumables disposed in the torch and an operator interface switch connected to the plasma control circuit and configured to initiate generation of a plasma arc from the plasma arc torch; and activating a switch, separate from the operator interface switch, disposed on the torch and connected to the plasma control circuit to disconnect the plasma control circuit to limit the current, including pilot current, from flowing to the one or more consumables.

Abstract: In some aspects, methods for controlling a pneumatic system in a plasma arc processing system can include: receiving, by a computing device, a command to begin a plasma processing operation; generating a valve command signal for a valve that includes an operational drive voltage of at least about 125% of a continuous duty cycle coil voltage rating of the valve to open the valve; and once open, adjusting the valve command signal to facilitate a steady state operation to: monitor a steady state operational duty cycle of the valve, the steady state operational duty cycle being determined by comparing the continuous duty cycle coil voltage rating of the valve to an actual operational drive voltage supplied to the valve, and control the operational drive voltage supplied to the valve to maintain a steady state operational duty cycle of the valve at less than about 60% during steady state operation.

Abstract: In some aspects, methods of disengaging a plasma control circuit within a plasma arc torch of a plasma processing system to place the torch into a safety-locked mode can include providing: a plasma control circuit configured to convey a current from the plasma cutting system through the plasma arc torch to one or more consumables disposed in the torch and an operator interface switch connected to the plasma control circuit and configured to initiate generation of a plasma arc from the plasma arc torch; and activating a switch, separate from the operator interface switch, disposed on the torch and connected to the plasma control circuit to disconnect the plasma control circuit to limit the current, including pilot current, from flowing to the one or more consumables.

Abstract: In some aspects, methods for controlling a plasma arc in a plasma torch of a plasma cutting system in a low operating current mode can include: receiving, by a computing device within the plasma power supply, a command to begin a plasma processing operation; generating a pilot arc command to generate a pilot arc within the plasma torch, the generating of the pilot arc command including directing an electrical signal and a gas flow to the plasma torch, the electrical signal being configured to generate the pilot arc at a current having a first arc amperage magnitude; and generating an operational arc command to facilitate a transition from the pilot arc to an operational plasma arc, the generating of the operational arc command including adjusting the current directed to the plasma torch to be a second arc amperage magnitude that is lower than the first arc amperage magnitude.

Abstract: In some aspects, methods for controlling a pneumatic system in a plasma arc processing system can include: receiving, by a computing device, a command to begin a plasma processing operation; generating a valve command signal for a valve that includes an operational drive voltage of at least about 125% of a continuous duty cycle coil voltage rating of the valve to open the valve; and once open, adjusting the valve command signal to facilitate a steady state operation to: monitor a steady state operational duty cycle of the valve, the steady state operational duty cycle being determined by comparing the continuous duty cycle coil voltage rating of the valve to an actual operational drive voltage supplied to the valve, and control the operational drive voltage supplied to the valve to maintain a steady state operational duty cycle of the valve at less than about 60% during steady state operation.

Abstract: A plasma arc cutting system includes a power supply and a plasma torch attachable to the power supply. The plasma torch generates a plasma arc for cutting a workpiece. A reader is associated with the plasma torch. The reader is capable of reading stored data from an identification device located on a cartridge or a consumable component of the plasma arc cutting system. A controller is within the plasma arc cutting system and in communication with the power supply. The controller is capable of automatically establishing operating parameters of the plasma arc cutting system based upon the data stored on the identification device. An override feature allows a user of the plasma arc cutting system to override the automatically established operating parameters of the torch and to input user selected operating parameters.

Abstract: The invention features methods and apparatuses for establishing operational settings of a plasma arc cutting system. A plasma power supply includes a user selectable control. The user selectable control enables selection of a single cutting persona that establishes at least a current, a gas pressure or gas flow rate, and an operational mode of the plasma arc cutting system.

Abstract: The invention features methods and apparatuses for establishing operational settings of a plasma arc cutting system. A plasma power supply includes a user selectable control. The user selectable control enables selection of a single cutting persona that establishes at least a current, a gas pressure or gas flow rate, and an operational mode of the plasma arc cutting system.

Abstract: A plasma arc cutting system includes a power supply and a plasma torch attachable to the power supply. The plasma torch generates a plasma arc for cutting a workpiece. A reader is associated with the plasma torch. The reader is capable of reading stored data from an identification device located on a cartridge or a consumable component of the plasma arc cutting system. A controller is within the plasma arc cutting system and in communication with the power supply. The controller is capable of automatically establishing operating parameters of the plasma arc cutting system based upon the data stored on the identification device. An override feature allows a user of the plasma arc cutting system to override the automatically established operating parameters of the torch and to input user selected operating parameters.

Abstract: A portable plasma cutting system includes a power supply and a torch attachable to the power supply. The torch generates a plasma arc for cutting a workpiece. A gas valve is disposed in the system or the torch. The gas valve establishes a gas flow rate or a gas pressure in the torch. A controller is within the system and in communication with the power supply. The controller is capable of automatically manipulating the gas valve to establish the gas flow rate or pressure based upon a predetermined operating condition. A user activated switch is in communication with the controller. The switch has a first setting which causes the controller to automatically establish the gas flow rate or pressure based upon the predetermined operating condition, and a second setting which causes the controller to establish one of a user-determined gas flow rate or a user-determined gas pressure.

Abstract: The invention features methods and apparatuses for establishing operational settings of a plasma arc cutting system automatically using replaceable cartridges. A replaceable cartridge for use with a plasma arc cutting system includes a housing, a connection mechanism for coupling the housing to a plasma arc torch, an arc constrictor connected to the housing, an arc emitter connected to the housing, and an identification mechanism disposed relative to the housing and configured to communicate information to a reader of the plasma arc cutting system and automatically set at least one operating parameter of the plasma arc cutting system.

Abstract: A portable plasma cutting system includes a power supply and a torch attachable to the power supply. The torch generates a plasma arc for cutting a workpiece. A gas valve is disposed in the system or the torch. The gas valve establishes a gas flow rate or a gas pressure in the torch. A controller is within the system and in communication with the power supply. The controller is capable of automatically manipulating the gas valve to establish the gas flow rate or pressure based upon a predetermined operating condition. A user activated switch is in communication with the controller. The switch has a first setting which causes the controller to automatically establish the gas flow rate or pressure based upon the predetermined operating condition, and a second setting which causes the controller to establish one of a user-determined gas flow rate or a user-determined gas pressure.