Abstract: Provided is a multi-functional integrated molecular test cartridge and application thereof. The multi-functional integrated molecular test cartridge includes a deformable assembly, a blocking assembly and a reaction assembly, where the blocking assembly is placed between the deformable assembly and the reaction assembly, and a channel in communication with the reaction assembly and the deformable assembly is formed on the blocking assembly. The multi-functional integrated molecular test cartridge can be used for isothermal amplification reactions such as cross priming amplification (CPA), recombinase polymerase amplification (RPA), loop-mediated isothermal amplification (LAMP) and rolling circle amplification (RCA), can achieve integrated pre-loading of all reagents, a whole-process integrated test from a sample to a result, and flexible sample processing modes, and has high biological safety and a low risk of product and sample leakage.

Abstract: A color sorting method for small-grain agricultural products combining an area scanning photoelectric characteristic and a line scanning photoelectric characteristic is provided. The present invention obtains an area scan image of small-grain agricultural product materials on a conveyor belt by using an area scan camera, which can accurately extract area array features of the materials and realize accurate identification of the unqualified materials. At the same time, the present invention can provide key parameters for accurate positioning during free falling of the materials while identifying the unqualified materials by using the area scan image, and can cooperate with the line scan positioning camera and the pneumatic nozzle to achieve high-speed elimination of the unqualified materials.

Abstract: A color sorting method for small-grain agricultural products combining an area scanning photoelectric characteristic and a line scanning photoelectric characteristic is provided. The present invention obtains an area scan image of small-grain agricultural product materials on a conveyor belt by using an area scan camera, which can accurately extract area array features of the materials and realize accurate identification of the unqualified materials. At the same time, the present invention can provide key parameters for accurate positioning during free falling of the materials while identifying the unqualified materials by using the area scan image, and can cooperate with the line scan positioning camera and the pneumatic nozzle to achieve high-speed elimination of the unqualified materials.

Abstract: An nucleic acid integrated detection reagent tube is provided, it includes a main tube and one or more branch tubes, wherein a lysing zone, a cleaning zone and a plurality of separation plugs comprising at least a least a separation plug and a second separation plug which are sequentially disposed, the first separation plug is used for separating the lysing zone and the cleaning zone, a reaction zone is provided in the branch tubes and the second separation plug is positioned at a connection between the branch tubes and the main tube or inside the branch tubes; hydrophobic layers in a liquid or a solid phase disposed at each of the plurality of separation plugs; and a magnetic bead channel for magnetically carrying the nucleic acid to sequentially pass through each hydrophobic layer penetrating to the branch tubes defined in an inner wall of the detection reagent tube.

Abstract: An nucleic acid integrated detection method is provided, the method includes separating a lysis solution, a cleaning solution and a reaction solution in a detection reagent tube by providing a plurality of separation plugs in an over-under arrangement and disposing a hydrophobic layer in liquid or solid phase on each separation plug; adding a sample into the lysis solution; extracting nucleic acid in the sample using magnetic nanobeads; and then driving the magnetic nanobeads carrying the nucleic acid to sequentially pass through each hydrophobic layer along a magnetic bead channel and into the cleaning solution and the reaction solution to realize a cleaning and amplification for the nucleic acid, and finally, detecting the nucleic acid of the sample by an external device using an optical detection method, thus realizing a plurality of steps of nucleic acid extraction, cleaning and amplification reactions in the same detection reagent tube.

Abstract: A nucleic acid integrated detection method and detection reagent tube are provided, separating a lysis solution, a cleaning solution and a reaction solution in a detection reagent tube by providing a plurality of separation plugs in an over-under arrangement and disposing a hydrophobic layer in liquid or solid phase on each separation plug; adding a sample into the lysis solution; extracting nucleic acid in the sample using magnetic nanobeads; and then driving the magnetic nanobeads carrying the nucleic acid to sequentially pass through each hydrophobic layer along a magnetic bead channel and into the cleaning solution and the reaction solution to realize a cleaning and amplification for the nucleic acid, and finally, detecting the nucleic acid of the sample by an external device using an optical detection method, thus realizing a plurality of steps of nucleic acid extraction, cleaning and amplification reactions in the same detection reagent tube.

Abstract: A nucleic acid integrated detection method and detection reagent tube are provided, separating a lysis solution, a cleaning solution and a reaction solution in a detection reagent tube by providing a plurality of separation plugs in an over-under arrangement and disposing a hydrophobic layer in liquid or solid phase on each separation plug; adding a sample into the lysis solution; extracting nucleic acid in the sample using magnetic nanobeads; and then driving the magnetic nanobeads carrying the nucleic acid to sequentially pass through each hydrophobic layer along a magnetic bead channel and into the cleaning solution and the reaction solution to realize a cleaning and amplification for the nucleic acid, and finally, detecting the nucleic acid of the sample by an external device using an optical detection method, thus realizing a plurality of steps of nucleic acid extraction, cleaning and amplification reactions in the same detection reagent tube.