Abstract: An anode catalyst layer of a direct liquid feed fuel cell includes a Pt—Ru or Pt—Pd black catalyst; and a supported Pt—Ru or Pt—Pd catalyst having Pt—Ru or Pt—Pd supported on a carbon-based support. A membrane-electrode assembly of a direct liquid feed fuel cell includes an electrolyte membrane; and anode and cathode electrodes positioned to face each other with the electrolyte membrane being positioned therebetween, wherein the anode and cathode electrodes respectively include a gas diffusion layer and a catalyst layer. The anode catalyst layer of a direct liquid feed fuel cell shows excellent activity for an oxidation reaction of fuel and good catalyst stability and durability together with minimizing a dose of catalyst since a black catalyst and a supported catalyst are used together at an optimized ratio.