Abstract: Provided is a self-assembling peptide, a preparation method, the self-assembling peptide formulations and use, and it is related to the field of biotechnology. The self-assembling peptide provided by the present disclosure has a general formula as shown in AC-Pro-X1-X3-X2-X3-X1-X3-X2-Pro-amide. Through experiments, the inventors of the present disclosure found that the self-assembling peptide provided by the present disclosure, because solubility is high and difficulty of synthesis and purification is less compared with a traditional self-assembling peptide, is more feasible for industrial production, and even cheaper to produce. In mass synthesis and purification, the number of purification decreases, the single purification amount increases, the purity of a crude peptide can reach 90% or more, and the cost is greatly reduced.