Abstract: The present invention discloses a fluorescent probe for detecting a sulfenated protein, which has good stability and can specifically quantitatively detect a sulfenated protein in complex biological samples, and has a good detection ability of signal-to-noise ratios, is highly sensitive, and has excellent selectivity, thereby realizing specific detection of sulfhydryl sulfenation modification of intracellular proteins.

Abstract: The present invention discloses a fluorescent probe for detecting a sulfenated protein, having the following structural general formula (I). The fluorescent probe has good stability and can be preserved for long-term use. The fluorescent probe is highly sensitive to its environment, having no strong fluorescence in a hydrophilic environment, and producing strong fluorescence only after reaction with a sulfenated protein such that a fluorophore is in a hydrophobic environment in the protein structure, and thus is able to develop in situ in cells the sulfhydryl sulfenated protein. Furthermore, the fluorescent probe has good water solubility and a relatively large absorption-emission wavelength difference, and can be paired with a tryptophan residue in a protein to produce a fluorescence resonance energy transfer (FRET) effect in an extracellular buffer solution detection system, effectively avoiding interference of excitation light and emitted light in protein samples.