Patents by Inventor Justin Andrew SCHARES
Justin Andrew SCHARES has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220110318Abstract: Liquid compositions for preserving and storing pollen, especially maize pollen, include pollen suspensions in solutions with particular pH ranges. Methods for preserving and storing pollen, especially maize pollen, include suspending pollen in solutions with particular pH ranges and storing them at particular temperatures.Type: ApplicationFiled: September 5, 2019Publication date: April 14, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: MAREN L ARLING, EMILY BERNTSON, KEVIN DAY, WILLIAM JAMES GORDON-KAMM, HENA GUO, JUSTIN ANDREW SCHARES, YUE YUN
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Patent number: 10477859Abstract: Methods for obtaining genetic material from plant embryos while preserving their viability are provided. In the methods, preservation of viability may be maintained by suspending the embryos in an aqueous solution surrounded by an oil matrix. Genetic material may be obtained from an aliquot of the aqueous solution and may be used directly for molecular analysis, or whole genome amplification may be performed prior to molecular analysis.Type: GrantFiled: June 4, 2015Date of Patent: November 19, 2019Assignee: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Randal Arnold, Matthew Paul Cope, Justin Andrew Schares, Yue Yun
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Publication number: 20190300970Abstract: Methods and compositions to genotype microspores and/or pollen grains are provided. Methods provided include using meiotically-related products to non-destructively determine the genotype of one of the meiotically-related products such that it can be further used. For example, methods to obtain a genotype of a microspore are provided. The methods include: isolating tetrads, separating the tetrads to obtain four tetrad microspores; genotyping three of the four tetrad microspores, and inferring the genotype of the fourth tetrad microspore. Methods are also provided for generating doubled haploid plants from the microspores that are selected for further analysis based on inferred genotypes. Viable genotyped pollen produced by these methods, as well as seed, cells, plants, germplasm, progeny, and plant parts derived therefrom are also provided.Type: ApplicationFiled: June 27, 2017Publication date: October 3, 2019Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: WAYNE MATTHEW CRISMANI, KEVIN DAY, GREGORY D MAY, JUSTIN ANDREW SCHARES, YUE YUN
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Patent number: 10278345Abstract: Methods for preserving viability of plant tissues such as plant embryos are provided herein. Also included are methods for storing genomic DNA and/or molecular marker assay materials in an oil bilayer as part of a high-throughput molecular characterization system. Moreover, plant embryos may be treated while in an oil matrix. The treatment may include chromosome doubling, Agrobacterium-mediated transformation, or herbicide selection as part of an embryo rescue process.Type: GrantFiled: August 26, 2016Date of Patent: May 7, 2019Assignee: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Randal Arnold, Roberto Barreiro, Matthew Paul Cope, Clifford P Hunter, Justin Andrew Schares, Xinli Emily Wu, Yue Yun
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Patent number: 10280472Abstract: Methods for obtaining genetic material from plant embryos while preserving their viability as well as methods for performing a molecular analysis of plant embryos, particularly with small quantities of genetic material by collecting shed cellular material from one or more plant embryos; obtaining DNA from the shed cellular material; performing a molecular analysis of the DNA; and germinating at least one of the one or more plant embryos. A further extension includes determining whether to germinate and grow the embryo or to discard the embryo based on its genotype as part of a breeding process. Also provided are methods of genotyping embryos using embryo shed cellular material contained in or on agar and methods of analyzing plant embryonic tissue derived from microspores.Type: GrantFiled: June 4, 2015Date of Patent: May 7, 2019Assignee: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Randal Arnold, Matthew Paul Cope, Justin Andrew Schares, Yue Yun
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Publication number: 20180016645Abstract: Methods and compositions to genotype microspores and/or pollen grains are provided. Methods provided include using meiotically-related products to non-destructively determine the genotype of one of the meiotically-related products such that it can be further used. For example, methods to obtain a genotype of a microspore are provided. The methods include: isolating tetrads, separating the tetrads to obtain four tetrad microspores; genotyping three of the four tetrad microspores, and inferring the genotype of the fourth tetrad microspore. Methods are also provided for generating doubled haploid plants from the microspores that are selected for further analysis based on inferred genotypes. Viable genotyped pollen produced by these methods, as well as seed, cells, plants, germplasm, progeny, and plant parts derived therefrom are also provided.Type: ApplicationFiled: July 15, 2016Publication date: January 18, 2018Inventors: WAYNE MATTHEW CRISMANI, KEVIN DAY, GREGORY D. MAY, JUSTIN ANDREW SCHARES, YUE YUN
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Publication number: 20170273261Abstract: Methods for preserving viability of plant tissues such as plant embryos are provided herein. Also included are methods for storing genomic DNA and/or molecular marker assay materials in an oil bilayer as part of a high-throughput molecular characterization system. Moreover, plant embryos may be treated while in an oil matrix. The treatment may include chromosome doubling, Agrobacterium-mediated transformation, or herbicide selection as part of an embryo rescue process.Type: ApplicationFiled: July 7, 2015Publication date: September 28, 2017Inventors: Randal Arnold, Roberto Barreiro, Matthew Paul Cope, Clifford Paul Hunter, Justin Andrew Schares, Xinli Emily Wu, Yue Yun
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Publication number: 20170273297Abstract: Methods for obtaining genetic material from plant embryos while preserving their viability are provided. In the methods, preservation of viability may be maintained by suspending the embryos in an aqueous solution surrounded by an oil matrix. Genetic material may be obtained from an aliquot of the aqueous solution and may be used directly for molecular analysis, or whole genome amplification may be performed prior to molecular analysis.Type: ApplicationFiled: June 4, 2015Publication date: September 28, 2017Inventors: Randal Arnold, Matthew Paul Cope, Justin Andrew Schares, Yue Yun
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Patent number: 9706723Abstract: Methods for preserving viability of plant tissues such as plant embryos are provided herein. Also included are methods for storing genomic DNA and/or molecular marker assay materials in an oil bilayer as part of a high-throughput molecular characterization system. Moreover, plant embryos may be treated while in an oil matrix. The treatment may include chromosome doubling, Agrobacterium-mediated transformation, or herbicide selection as part of an embryo rescue process.Type: GrantFiled: July 7, 2015Date of Patent: July 18, 2017Assignee: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Randal Arnold, Roberto Barreiro, Matthew Paul Cope, Clifford P Hunter, Justin Andrew Schares, Xinli Emily Wu, Yue Yun
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Publication number: 20170006766Abstract: Methods for preserving viability of plant tissues such as plant embryos are provided herein. Also included are methods for storing genomic DNA and/or molecular marker assay materials in an oil bilayer as part of a high-throughput molecular characterization system. Moreover, plant embryos may be treated while in an oil matrix. The treatment may include chromosome doubling, Agrobacterium-mediated transformation, or herbicide selection as part of an embryo rescue process.Type: ApplicationFiled: July 7, 2015Publication date: January 12, 2017Inventors: RANDAL ARNOLD, Roberto Barreiro, Matthew Paul Cope, Clifford P Hunter, Justin Andrew Schares, Xinli Emily Wu, Yue Yun
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Publication number: 20160360716Abstract: Methods for preserving viability of plant tissues such as plant embryos are provided herein. Also included are methods for storing genomic DNA and/or molecular marker assay materials in an oil bilayer as part of a high-throughput molecular characterization system. Moreover, plant embryos may be treated while in an oil matrix. The treatment may include chromosome doubling, Agrobacterium-mediated transformation, or herbicide selection as part of an embryo rescue process.Type: ApplicationFiled: August 26, 2016Publication date: December 15, 2016Inventors: RANDAL ARNOLD, ROBERTO BARREIRO, MATTHEW PAUL COPE, CLIFFORD P. HUNTER, JUSTIN ANDREW SCHARES, XINLI EMILY WU, YUE YUN
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Publication number: 20160060714Abstract: The invention provides methods for obtaining genetic material from plant embryos while preserving their viability as well as methods for performing a molecular analysis of plant embryos, particularly with small quantities of genetic material. The methods may include the steps of collecting shed cellular material from one or more plant embryos; obtaining DNA from the shed cellular material; performing a molecular analysis of the DNA; and germinating at least one of said one or more plant embryos. A further extension of this method includes determining whether to germinate and grow the embryo or to discard the embryo based on its genotype as part of a breeding process. Also provided are methods of genotyping embryos using embryo shed cellular material contained in or on agar and methods of analyzing plant embryonic tissue derived from microspores.Type: ApplicationFiled: June 4, 2015Publication date: March 3, 2016Inventors: Randal ARNOLD, Matthew Paul COPE, Justin Andrew SCHARES, Yue YUN
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Publication number: 20160060715Abstract: The invention provides methods for obtaining genetic material from plant embryos while preserving their viability as well as methods for performing a molecular analysis of plant embryos, particularly with small quantities of genetic material. The methods may include the steps of collecting shed cellular material from one or more plant embryos; obtaining DNA from the shed cellular material; performing a molecular analysis of the DNA; and germinating at least one of said one or more plant embryos. A further extension of this method includes determining whether to germinate and grow the embryo or to discard the embryo based on its genotype as part of a breeding process. Also provided are methods of genotyping embryos using embryo shed cellular material contained in or on agar and methods of analyzing plant embryonic tissue derived from microspores.Type: ApplicationFiled: June 4, 2015Publication date: March 3, 2016Inventors: Randal ARNOLD, Matthew Paul COPE, Justin Andrew SCHARES, Yue YUN