Abstract: Disclosed herein are processes for reducing emulsion during the process of obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by maintaining the level of carbohydrate at less than 15 g/Kg in the fermentation broth. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.