Abstract: Methods, systems, and compositions for non-invasively detecting and/or monitoring therapeutic nucleic acid constructs in a sample comprising cell-free nucleic acids from a subject. Detection of therapeutic nucleic acid constructs in samples comprising cell-free nucleic acids allows for verifying therapeutic nucleic acid construct administration, determining the persistence or biological efficacy of the therapeutic nucleic acid construct, and/or ascertaining the efficacy of the therapy in the subject.

Abstract: Provided herein are methods of determining a molecular response score. The molecular response score may be used to monitor and guide administration of treatment to a subject.

Abstract: Provided herein are methods that are useful in determining the cellular origin of cell-free nucleic acid (cfNA) fragments from cfNA samples, such as liquid biopsy samples. The methods disclosed herein typically improve the specificity and/or sensitivity of assays for detecting diseased cell nucleic acids (e.g., cancer cell DNA) in cfNA samples by identifying variant alleles produced by non-target cells, such as hematopoietic stem cells, in certain embodiments. Yet other aspects include related systems and computer readable media, among numerous other applications.

Abstract: Methods, systems, and compositions for non-invasively detecting and/or monitoring therapeutic nucleic acid constructs in a sample comprising cell-free nucleic acids from a subject. Detection of therapeutic nucleic acid constructs in samples comprising cell-free nucleic acids allows for verifying therapeutic nucleic acid construct administration, determining the persistence or biological efficacy of the therapeutic nucleic acid construct, and/or ascertaining the efficacy of the therapy in the subject.