Abstract: A method of producing serum or plasma comprises the steps of drawing a blood sample from a subject; adding erythrocyte sedimentation-accelerating agent(s) and an anticoagulant to the blood sample; placing the mixture in a vessel with an inclined well and allowing the phase separation of the solution into an erythrocyte phase and a plasma or serum phase, producing an erythrocyte-depleted serum or plasma; and collecting the erythrocyte-depleted serum or plasma.

Abstract: The present disclosure relates to methods for preparing car cartilage for eyelid reconstruction, including upper eyelid reconstruction, after trauma, disease, or tumor excision. The present disclosure also relates to compositions, uses, and methods for reconstructing an eyelid. The present disclosure also relates to kits for preparing ear cartilage for eyelid reconstruction. The disclosed compositions, uses, kits, and methods may also be used for autograft, allograft, isograft, and xenograft transplantation, for stored or banked grafts, and for other therapeutic uses.