Abstract: A food tenderizer sleeve and method of use is provided. The food tenderizer sleeve includes two end collars and segmented and split cutting blade sleeves, which are easy to remove, replace, and clean. In some embodiments, the segments of the cutting blade sleeves form generally a half-circle cross-sectional shape and mount a plurality of cutting blades and spacers between the cutting blades. In some embodiments, the segments include one end with male connection tabs and a second end with female tab receivers for connection to another segment or to an end collar. Each cutting blade sleeve segment is further configured for matching up with another sleeve segment to form a tube or sleeve shape configured to fit around a rotational drive shaft.

Abstract: A display bag is provided and may include a housing formed from a flexible material and having a first end, a second end, and an inner volume extending between the first end and the second end. The display bag may also include a hanging device disposed proximate to the first end and a first panel disposed proximate to the second end and movable from a closed state closing a first opening formed in the housing and restricting access to the inner volume and an open state permitting access to the inner volume via the first opening.

Abstract: The present invention provides a method for purifying a protein complex and the components comprising the complex from a cell, a cell or tissue lysate or a whole organism by employing a combined set of affinity tags of high affinity, specificity and ease of elution. The method involves using a protein or a peptide modified to contain one or more affinity tags separated by one or more specific protease cleavage sites to isolate any interacting proteins or fragments thereof. Specifically exemplified is a method employing a modified bait containing AviTag or GST and a removal tag of His6 hexapeptide positioned proximal to the bait such that any excess bait can be efficiently removed from the purified complex yielding enriched interacting proteins prior to subjecting the complex for further identification of individual components. The identification of the proteins or fragments thereof contained in the protein complex will provide new targets for the identification of new pharmaceuticals and diagnostic tools.

Abstract: The present invention provides a method for purifying a protein complex and the components comprising the complex from a cell, a cell or tissue lysate or a whole organism by employing a combined set of affinity tags of high affinity, specificity and ease of elution. The method involves using a protein or a peptide modified to contain one or more affinity tags separated by one or more specific protease cleavage sites to isolate any interacting proteins or fragments thereof. Specifically exemplified is a method employing a modified bait containing AviTag or GST and a removal tag of His6 hexapeptide positioned proximal to the bait such that any excess bait can be efficiently removed from the purified complex yielding enriched interacting proteins prior to subjecting the complex for further identification of individual components. The identification of the proteins or fragments thereof contained in the protein complex will provide new targets for the identification of new pharmaceuticals and diagnostic tools.