Abstract: The present invention provides a novel culture medium and method for culturing stem cells, preferably muscle-derived mesenchymal stem cells. The culture medium comprises clotted blood plasma at 5-70% in base medium. The cultured cells may be released without use of trypsin by resolubilizing the clotted blood plasma with the assistance of proteolytic enzymes or anticoagulation agents.

Abstract: The present invention relates to a method for preparing differentiated mammalian cells comprising collecting muscle microbiopsy, placing the sample in a cell culture medium, collecting and growing the cells and differentiating them in suitable differentiation medium.

Abstract: The present invention relates to a method for assessing the effects of a compound or composition on a mammal, comprising obtaining mammalian pluripotent muscle-derived mesenchymal stem cells or a population thereof, exposing the said stem cells, stem cell population to the relevant compound or composition, and assessing the effects on the said cells. The invention method is a reliable assessment method applicable to drug development, toxicity testing and optimized personalized medicine.

Abstract: The present invention provides a new method of obtaining muscle-derived mesenchymal stem cells from microbiopsies of mammalian origin. The invention provides for a minimally invasive methodology yielding high amounts of MSCs that can differentiate into different cell lineages.

Abstract: The invention relates to a multi-site production system for production of a product under regulated conditions comprising at least two production sites (12) linked to a central monitoring unit (10), each production site (12) comprising at least one production unit (16) for production under regulated conditions and a computerized control unit (14) designed to receive, possibly record, and transfer required product and/or process parameters to the central monitoring unit (10) designed to check for validation purposes the production process and/or product quality. The invention further relates to a method for multi-site production under regulated conditions making use of above system.

Abstract: The present invention provides a new method of obtaining muscle-derived mesenchymal stem cells from microbiopsies of mammalian origin. The invention provides for a minimally invasive methodology yielding high amounts of MSCs that can differentiate into different cell lineages.