Abstract: The present invention provides a biochip substrate used for immobilizing biomaterials, which has a matrix and at least one metal oxide thread-like nano-object formed vertically on a surface of the matrix. The metal oxide thread-like nano-object is employed for immobilizing the biomaterial onto the matrix of the chip substrate directly, without complicated immobilizing processes by using cross-linking agents or curing agents, and thereby simplifying the process of fabrication. Arrangement as such makes it suitable for purification of a biomaterial. On the other hand, the metal oxide thread-like nano-object characterized by having an enormous surface area is suitable for fabricating immunosensor chip and capable of maintaining high activity and reactivity of biomaterials, so as to increase speed and yield of reactions.

Abstract: This invention relates to a method for immunoassays based on infrared reflection absorption spectroscopy that utilizes proteins such as antigen and antibody that have specific absorptions in infrared absorption spectrum under 1550 cm?1. A chip is made from infrared reflective substrates such as a gold or silver plate, whereon the antibodies or antigens are immobilized by the specific bonding action between metal surfaces of the chip and proteins, and the protein signals are then detected by infrared reflection-absorption and infrared microscopy. Since infrared radiation is capable of interacting with any organic molecules, there is no need to label the samples with fluorescent reagents or nucleic irradiation reagent. This invention thus provides a label-free process that can avoid the inconvenience of labeling common biochemical material for detection in the prior art, thus achieving the purpose of higher speed of detection.

Abstract: This invention relates to a method for immunoassays based on infrared reflection absorption spectroscopy that utilizes proteins such as antigen and antibody that have specific absorptions in infrared absorption spectrum under 1550 cm−1. A chip is made from infrared reflective substrates such as a gold or silver plate, whereon the antibodies or antigens are immobilized by the specific bonding action between metal surfaces of the chip and proteins, and the protein signals are then detected by infrared reflection-absorption and infrared microscopy. Since infrared radiation is capable of interacting with any organic molecules, there is no need to label the samples with fluorescent reagents or nucleic irradiation reagent. This invention thus provides a label-free process that can avoid the inconvenience of labeling common biochemical material for detection in the prior art, thus achieving the purpose of higher speed of detection.