Abstract: The invention provides methods of detecting activation of an immune cell, methods for detecting blocking or enhancing properties of a test reagent or stimuli on activation of one or more immune cells or platelets, methods for selecting hybridomas producing antibodies to an antigen for quality of strength of binding to the antigen, methods for determining if a subject has had an immune response to an immunogen, methods of isolating neutralizing antibodies for an immunogen, methods of classifying a B cell lymphoma, and methods for selecting activated B-cells expressing an antibody to one or more antigens.