Abstract: A target DNA sequence (1) is contacted with ML+MR ligation oligonucleotides (10, 20) under hybridization conditions. The ligation oligonucleotides (10, 20) comprises a respective UMI (14, 15). A ligating agent ligates together the ligation oligonucleotides (10, 20) while hybridized to the target DNA sequence (1) to form a ligated product (30). The ligated product (30) is amplified by means to amplification primers (40, 50), of which one comprises a sample-specific barcode sequence (55), to form an amplified product (60) comprising two UMIs (65), a sequence of interest (66) and one barcode sequence (68). Amplified products (60) from multiple samples are pooled together, sequenced, demultiplexed and mapped to enable quantification of unique target DNA sequences (1) in the different samples.

Abstract: A blocking oligonucleotide (10, 10A, 10A?, 10A?, 10B, 10C, 10D, 10E) comprises a 3?-end complementary sequence (13) complementary to a 3?-end sequence (23) of a globin mRNA molecule (20; 20A, 20B) and a poly-A complementary sequence (12) of at least one nucleotide complementary to at least a portion of a poly-A sequence (22) of the globin mRNA molecule (20, 20A, 20B). The blocking oligonucleotide (10, 10A, 10A?, 10A?, 10B, 10C, 10D, 10E) is capable of inhibiting binding of a reverse transcription anchored poly-T primer (30) to the globin mRNA molecule (20, 20A, 20B) and thereby significantly reducing synthesis of globin cDNA from globin mRNA molecules (20, 20A, 20B) present in a sample. This high reduction of globin cDNA by the blocking oligonucleotide (10, 10A, 10A?, 10A?, 10B, 10C, 10D, 10E) is achieved without any significant degradation of mRNA molecules present in the sample.

Abstract: A genotyping method and a prepared oligomicroarray as device is to determine single nucleotide polymorphism (SNP) and mutations are provided. The method uses two specific APEX-2 primers per each SNP or mutations to be determined. The same primers are used in amplification phase (primer extension and PCR with universal primer) and in the single base extension phase on an array. All SNP-containing sequences can be genotyped and amplified in one reaction tube and visualized on a microarray.

Abstract: A genotyping method and a prepared oligomicroarray as device is to determine single nucleotide polymorphism (SNP) and mutations are provided. The method uses two specific APEX-2 primers per each SNP or mutations to be determined. The same primers are used in amplification phase (primer extension and PCR with universal primer) and in the single base extension phase on an array. All SNP-containing sequences can be genotyped and amplified in one reaction tube and visualized on a microarray.