Abstract: An Image Activated Cell Sorting (IACS) classification workflow includes: employing a neural network-based feature encoder (or extractor) to extract features of cell images; automatically clustering cells based on extracted cell features; identifying a cluster to pick which cluster(s) to sort based on the cell images; fine-tuning a classification network based on the cluster(s) selected; and once refined, the classification network is used to sort cells for real-time live sorting.

Abstract: A method for synthesizing 9,10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide or its derivatives has a step of introducing 6-chloro-6H-dibenz[c,e][1,2]oxaphosphorin or its derivative, an acid compound and water into a reacting chamber to form an organic layer having 9,10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide or its derivative and an aqueous layer. Because the acid compound is from an external source and has a catalyzing effect, employing the method can prevent side reaction from occurring and increase yield of 9,10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide or its derivative. Furthermore, the method is a one-pot operation of hydrolysis, dehydration and cyclization, so the method does not require purification of intermediates. Therefore, the method is time- and cost-saving and requires less organic solvent, resulting in less pollution to the environment.

Abstract: A method for synthesizing 9,10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide or its derivatives has a step of introducing 6-chloro-6H-dibenz[c,e][1,2]oxaphosphorin or its derivative, an acid compound and water into a reacting chamber to form an organic layer having 9,10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide or its derivative and an aqueous layer. Because the acid compound is from an external source and has a catalyzing effect, employing the method can prevent side reaction from occurring and increase yield of 9,10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide or its derivative. Furthermore, the method is a one-pot operation of hydrolysis, dehydration and cyclization, so the method does not require purification of intermediates. Therefore, the method is time-and cost-saving and requires less organic solvent, resulting in less pollution to the environment.

Abstract: A method for synthesizing 4,4-dihalogen-2-(dialkylamino)methylene-3-oxy-alkylbutyrate and its derivatives has acts of: (a) providing N,N-dialkylamino-alkyl-acrylate in a reaction vessel; (b) adding organic alkali, organic solvent and multiple molecular sieves into the reaction vessel; (c) mixing N,N-dialkylamino-alkyl-acrylate, the organic alkali, the organic solvent and the molecular sieves; and (d) adding 2,2-dihalogen-acetyl-chloride into the reaction vessel and allowing a synthetic reaction of 2,2-dihalogen-acetyl-chloride and N,N-dialkylamino-alkyl-acrylate to obtain 4,4-dihalogen-2-(dialkylamino)methylene-3-oxy-alkylbutyrate. The molecular sieves are able to remove water efficiently to prevent materials in the reaction vessel from undergoing side reactions or changing chemical properties. Furthermore, the molecular sieves are able to adsorb chloride to avoid polymerization reactions for improved yield.

Abstract: A container assembly has a raw-material container and a product-collection container, which are heat resistant and pressure resistant and have graduations formed on a sidewall thereof and a joint protruding therefrom. The product-collection container is detachably mounted on the raw-material container and communicates with the raw-material container through the joint. Since the raw-material container has graduations, an amount of raw materials can be consistently added in each batch, therefore, conditions of sublimation such as pressure, temperature or the like do not require adjustment and may just be monitored to ensure maximum yield is attained. Therefore, a sublimation procedure is simple, saves time and decreases product costs. Since, the product-collection container has graduations, an amount of product can be observed easily by the graduations and the product is easily removed without removing impure byproducts. Therefore, purity of the product can be increased.