Patents by Inventor Kai Lao
Kai Lao has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11972519Abstract: Described herein are techniques for learning neural reflectance shaders from images. A set of one or more machine learning models can be trained to optimize an illumination latent code and a set of reflectance latent codes for an object within a set of input images. A shader can then be generated based on a machine learning model of the one or more machine learning models. The shader is configured to sample the illumination latent code and the set of reflectance latent codes for the object. A 3D representation of the object can be rendered using the generated shader.Type: GrantFiled: June 24, 2022Date of Patent: April 30, 2024Assignee: Intel CorporationInventors: Benjamin Ummenhofer, Shenlong Wang, Sanskar Agrawal, Yixing Lao, Kai Zhang, Stephan Richter, Vladlen Koltun
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Patent number: 10913976Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.Type: GrantFiled: March 12, 2019Date of Patent: February 9, 2021Assignee: Life Technologies CorporationInventors: Bin Li, Kai Lao, Jennifer O'Neil, Jennifer Kunkel, Kellie Haley, Rachel Kasinskas, Zhaochun Ma, Pius Brzoska
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Patent number: 10501784Abstract: The present disclosure relates to the amplification of target nucleic acid sequences for various sequencing and/or identification techniques. The use of these primers, as described herein, allows for the reduction in the amplification of nonspecific hybridization events (such as primer dimerization) while allowing for the amplification of the target nucleic acid sequences.Type: GrantFiled: August 15, 2016Date of Patent: December 10, 2019Assignee: Life Technologies CorporationInventors: Kai Lao, Neil Straus, Nanlan Xu
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Publication number: 20190264272Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.Type: ApplicationFiled: March 12, 2019Publication date: August 29, 2019Inventors: Bin LI, Kai Lao, Jennifer O'NEIL, Jennifer KUNKEL, Kellie HALEY, Rachel KASINSKAS, Zhaochun MA, Pius BRZOSKA
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Publication number: 20170107567Abstract: The present disclosure relates to the amplification of target nucleic acid sequences for various sequencing and/or identification techniques. The use of these primers, as described herein, allows for the reduction in the amplification of nonspecific hybridization events (such as primer dimerization) while allowing for the amplification of the target nucleic acid sequences.Type: ApplicationFiled: August 15, 2016Publication date: April 20, 2017Inventors: Kai LAO, Neil STRAUS, Nanlan XU
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Patent number: 9567635Abstract: The present teachings provide methods, compositions, and kits for performing primer extension reactions. In some embodiments, a reverse transcription reaction is performed on a target polynucleotide with a hot start primer comprising a blunt-ended self-complementary stem, and a loop, and extension products form at high temperatures but reduce extension product formation at low temperatures.Type: GrantFiled: March 12, 2015Date of Patent: February 14, 2017Assignee: Life Technologies CorporationInventors: Kai Lao, Neil Straus, Kenneth Livak
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Patent number: 9528147Abstract: The present disclosure relates to the amplification of target nucleic acid sequences. This can be accomplished via the use of various primers. The use of these primers, as described herein, results in nucleic acid structures that can reduce the amplification of nonspecific hybridization events (such as primer dimerization) while allowing the amplification of the target nucleic acid sequences.Type: GrantFiled: May 27, 2015Date of Patent: December 27, 2016Assignee: Life Technologies CorporationInventors: Kai Lao, Neil Straus
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Patent number: 9447463Abstract: The present disclosure relates to the amplification of target nucleic acid sequences for various sequencing and/or identification techniques. The use of these primers, as described herein, allows for the reduction in the amplification of nonspecific hybridization events (such as primer dimerization) while allowing for the amplification of the target nucleic acid sequences.Type: GrantFiled: December 9, 2013Date of Patent: September 20, 2016Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Kai Lao, Neil Straus, Nanlan Xu
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Publication number: 20150322504Abstract: The present disclosure relates to the amplification of target nucleic acid sequences. This can be accomplished via the use of various primers. The use of these primers, as described herein, results in nucleic acid structures that can reduce the amplification of nonspecific hybridization events (such as primer dimerization) while allowing the amplification of the target nucleic acid sequences.Type: ApplicationFiled: May 27, 2015Publication date: November 12, 2015Inventors: Kai LAO, Neil STRAUS
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Publication number: 20150252418Abstract: The present teachings provide methods, compositions, and kits for performing primer extension reactions. In some embodiments, a reverse transcription reaction is performed on a target polynucleotide with a hot start primer comprising a blunt-ended self-complementary stem, and a loop, and extension products form at high temperatures but reduce extension product formation at low temperatures.Type: ApplicationFiled: March 12, 2015Publication date: September 10, 2015Inventors: Kai LAO, Neil Straus, Kenneth Livak
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Patent number: 8962254Abstract: The present teachings provide methods, compositions, and kits for performing primer extension reactions on at least two target polynucleotides in the same reaction mixture. In some embodiments, a reverse transcription reaction is performed on a first target polynucleotide with a hot start primer comprising a self-complementary stem and a loop, and extension products form at high temperatures but extension products form less so at low temperatures since the self-complementary stem of the hot start primer prevents hybridization of the target specific region to the target. However, non-hot start primers with free target specific regions can hybridize to their corresponding targets at the low temperature and extension can happen at the low temperature.Type: GrantFiled: July 15, 2013Date of Patent: February 24, 2015Assignee: Applied Biosystems, LLCInventors: Kai Lao, Neil Straus
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Publication number: 20140349301Abstract: The present teachings are generally directed to methods for normalizing at least one species of small nucleic acid that is present in a population of small nucleic acid species, wherein the relative concentration of at least one small nucleic acid species is substantially greater than the relative concentration of at least one other small nucleic acid species in the population. At least one small nucleic acid species is normalized using a multiplicity of primers comprising degenerate sequences. In some embodiments, a small nucleic acid species is identified by inserting at least part of an extension product from a normalized population into a vector and subsequently sequencing the insert. In some embodiments, a small nucleic acid species is identified by determining the sequence of at least part of an extension product.Type: ApplicationFiled: June 2, 2014Publication date: November 27, 2014Applicant: APPLIED BIOSYSTEMS, LLCInventors: Kai LAO, Neil Straus, John Burns
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Publication number: 20140303017Abstract: The present teachings provide methods, compositions, and kits for reverse transcribing and amplifying small nucleic acids such as micro RNAs. High levels of multiplexing are provided by the use of a zip-coded stem-loop reverse transcription primer, along with a PCR-based pre-amplification reaction that comprises a zip-coded forward primer. Detector probes in downstream decoding PCRs can take advantage of the zip-code introduced by the stem-loop reverse transcription primer. In some embodiments, further amplification is achieved by cycling the reverse transcription reaction. The present teachings also provide compositions and kits useful for performing the reverse transcription and amplification reactions described herein.Type: ApplicationFiled: March 6, 2014Publication date: October 9, 2014Applicant: APPLIED BIOSYSTEMS, LLCInventors: Kai LAO, Kenneth Livak, Neil Straus
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Publication number: 20140235845Abstract: The present disclosure relates to the amplification of target nucleic acid sequences. This can be accomplished via the use of various primers. The use of these primers, as described herein, results in nucleic acid structures that can reduce the amplification of nonspecific hybridization events (such as primer dimerization) while allowing the amplification of the target nucleic acid sequences.Type: ApplicationFiled: February 10, 2014Publication date: August 21, 2014Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: KAI LAO, NEIL STRAUS
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Publication number: 20140206000Abstract: The present disclosure relates to the amplification of target nucleic acid sequences for various sequencing and/or identification techniques. The use of these primers, as described herein, allows for the reduction in the amplification of nonspecific hybridization events (such as primer dimerization) while allowing for the amplification of the target nucleic acid sequences.Type: ApplicationFiled: December 9, 2013Publication date: July 24, 2014Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Kai LAO, Neil Straus, Nanlan Xu
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Patent number: 8741569Abstract: The present teachings are generally directed to methods for normalizing at least one species of small nucleic acid that is present in a population of small nucleic acid species, wherein the relative concentration of at least one small nucleic acid species is substantially greater than the relative concentration of at least one other small nucleic acid species in the population. At least one small nucleic acid species is normalized using a multiplicity of primers comprising degenerate sequences. In some embodiments, a small nucleic acid species is identified by inserting at least part of an extension product from a normalized population into a vector and subsequently sequencing the insert. In some embodiments, a small nucleic acid species is identified by determining the sequence of at least part of an extension product.Type: GrantFiled: October 23, 2009Date of Patent: June 3, 2014Assignee: Applied Biosystems, LLCInventors: Kai Lao, Neil Straus, John Burns
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Publication number: 20140147845Abstract: The present teachings relate to methods, compositions, and kits for detecting one or more target polynucleotide sequences in a sample, and methods compositions and kits for forming concatameric ligation products. In some embodiments of the present teachings, oligonucleotides are hybridized to complementary target polynucleotides and are ligated together to form a concatameric ligation product. In some embodiments of the present teachings, the concatameric ligation product can be amplified, and the identity and quantity of the target polynucleotides determined based on sequence introduced in the ligation reaction. Some embodiments of the present teachings provide methods for removing unligated probes from the reaction mixture. Some embodiments of the present teachings provide for highly multiplexed detection, identification, and quantification of a plurality of target polynucleotides using a variety of analytical procedures.Type: ApplicationFiled: October 24, 2013Publication date: May 29, 2014Applicant: APPLIED BIOSYSTEMS, LLCInventors: Caifu CHEN, Kevin HENNESSY, Kai LAO, Teodoro PANER, Vinod MIRCHANDANI
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Publication number: 20140065624Abstract: The present teachings provide methods, compositions, and kits for performing primer extension reactions on at least two target polynucleotides in the same reaction mixture. In some embodiments, a reverse transcription reaction is performed on a first target polynucleotide with a hot start primer comprising a self-complementary stem and a loop, and extension products form at high temperatures but extension products form less so at low temperatures since the self-complementary stem of the hot start primer prevents hybridization of the target specific region to the target. However, non-hot start primers with free target specific regions can hybridize to their corresponding targets at the low temperature and extension can happen at the low temperature.Type: ApplicationFiled: July 15, 2013Publication date: March 6, 2014Inventors: Kai LAO, Neil STRAUS
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Patent number: 8487085Abstract: The present teachings provide methods, compositions, and kits for performing primer extension reactions on at least two target polynucleotides in the same reaction mixture. In some embodiments, a reverse transcription reaction is performed on a first target polynucleotide with a hot start primer comprising a self-complementary stem and a loop, and extension products form at high temperatures but extension products form less so at low temperatures since the self-complementary stem of the hot start primer prevents hybridization of the target specific region to the target. However, non-hot start primers with free target specific regions can hybridize to their corresponding targets at the low temperature and extension can happen at the low temperature.Type: GrantFiled: May 17, 2010Date of Patent: July 16, 2013Assignee: Applied Biosystems, LLCInventors: Kai Lao, Neil Straus
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Patent number: 8349563Abstract: The present disclosure relates to the amplification of target nucleic acid sequences for various sequencing and/or identification techniques. This can be accomplished via the use of target primers. The use of these target primers, as described herein, allows for the reduction in the amplification of undesired hybridization events (such as primer dimerization) while allowing for the amplification of the target nucleic acid sequences.Type: GrantFiled: October 16, 2009Date of Patent: January 8, 2013Assignee: Life Technologies CorporationInventors: Kai Lao, Neil Straus, Nanlan Xu