Patents by Inventor Kai Peter Johnsson

Kai Peter Johnsson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Sensors, methods and kits for detecting NADPH based on resonance energy transfer
    Patent number: 11959121
    Abstract: The invention relates to the detection of the cofactor reduced nicotinamide adenine dinucleotide phosphate (NADPH). Provided is a sensor molecule for the resonance energy transfer (RET)-based detection of NADPH, the sensor comprising a segment A connected via a linker to a segment B, wherein each of segment A and segment B comprises a member of a RET pair comprising a donor moiety and an acceptor moiety, further characterized in that (i) segment A comprises a binding protein (BP) for NADPH, the BP being dihydrofolate reductase (DHFR; EC 1.5.1.3) or a functional homolog, fragment, derivative or variant thereof, showing the desired NADPH binding properties, and wherein the BP comprises a heterologous protein domain inserted at or replacing at least part of the region corresponding to positions (20) to (27) of E.
    Type: Grant
    Filed: May 29, 2018
    Date of Patent: April 16, 2024
    Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.
    Inventors: Qiuliyang Yu, Kai Peter Johnsson
  • SENSORS, METHODS AND KITS FOR DETECTING NADPH BASED ON RESONANCE ENERGY TRANSFER
    Publication number: 20200181673
    Abstract: The invention relates to the detection of the cofactor reduced nicotinamide adenine dinucleotide phosphate (NADPH). Provided is a sensor molecule for the resonance energy transfer (RET)-based detection of NADPH, the sensor comprising a segment A connected via a linker to a segment B, wherein each of segment A and segment B comprises a member of a RET pair comprising a donor moiety and an acceptor moiety, further characterized in that (i) segment A comprises a binding protein (BP) for NADPH, the BP being dihydrofolate reductase (DHFR; EC 1.5.1.3) or a functional homolog, fragment, derivative or variant thereof, showing the desired NADPH binding properties, and wherein the BP comprises a heterologous protein domain inserted at or replacing at least part of the region corresponding to positions (20) to (27) of E.
    Type: Application
    Filed: May 29, 2018
    Publication date: June 11, 2020
    Inventors: Qiuliyang YU, Kai Peter JOHNSSON
  • Sensors, methods and kits for detecting nicotinamide adenine dinucleotides
    Patent number: 10221439
    Abstract: The invention relates to the in vitro and in cellulo detection of the cofactors nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP). Provided is a sensor molecule for fluorescence- or luminescence-based detection of a nicotinamide adenine dinucleotide analyte, in particular for detecting the concentrations of NAD+, NADP+ and/or the ratios of the concentrations of NAD+/NADH and NADP+/NADPH, the sensor comprising (i) a binding protein (BP) for the nicotinamide adenine dinucleotide analyte, the BP being derived from sepiapterin reductase (SPR; EC 1.1.1.153) (ii) an SPR ligand (SPR-L) capable of intramolecular binding to said BP in the presence of the oxidized form of said analyte; and (iii) at least one fluorophore.
    Type: Grant
    Filed: February 16, 2016
    Date of Patent: March 5, 2019
    Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.
    Inventors: Olivier Sallin, Luc Reymond, Kai Peter Johnsson
  • Sepiapterin reductase inhibitors
    Patent number: 9963462
    Abstract: Inhibitors of sepiapterin reductase and uses of sepiapterin reductase inhibitors in analgesia, treatment of acute and chronic pain, anti-inflammation, and immune cell regulation are disclosed.
    Type: Grant
    Filed: September 30, 2016
    Date of Patent: May 8, 2018
    Assignee: MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN E.V.
    Inventors: Mark Joseph Tebbe, Holly Victoria Atton, Craig Avery, Steven Mark Bromidge, Mark Kerry, Adrian Kotei Kotey, Nathaniel J. Monck, Mirco Meniconi, Mark Peter Ridgill, Heather Tye, Eddine Saiah, Kai Peter Johnsson, Katarzyna Irena Gorska, Hairuo Peng, John Michael McCall
  • Means and methods for bioluminescence resonance energy transfer (BRET) analysis in a biological sample
    Patent number: 9952208
    Abstract: The invention relates to the field of in vitro detection methods using luminescence. Provided is a sensor molecule for detecting an analyte of interest in a sample using bioluminescence resonance energy transfer (BRET), the sensor molecule comprising a proteinaceous moiety tethered to a synthetic regulatory molecule. Also provided is an analytical device comprising a sensor and methods using the sensor molecule.
    Type: Grant
    Filed: July 18, 2013
    Date of Patent: April 24, 2018
    Assignee: Ecole Polytechnique Fédérale de Lausanne
    Inventors: Kai Peter Johnsson, Alberto Schena, Rudolf Griss
  • Sensors, Methods and Kits for Detecting Nicotinamide Adenine Dinucleotides
    Publication number: 20180087083
    Abstract: The invention relates to the in vitro and in cellulo detection of the cofactors nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP). Provided is a sensor molecule for fluorescence or luminescence-based detection of a nicotinamide adenine dinucleotide analyte, in particular for detecting the concentrations of NAD+, NADP? and/or the ratios of the concentrations of NAD?/NAD H and NADP?/NADPH, the sensor comprising (i) a binding protein (BP) for the nicotinamide adenine dinucleotide analyte, the BP being derived from sepiapterin reductase (SPR; EC 1.1.1.153) (ii) a SPR ligand (SPR-L) capable of intramolecular binding to said BP in the presence of the oxidized form of said analyte; and (iii) at least one fluorophore.
    Type: Application
    Filed: February 16, 2016
    Publication date: March 29, 2018
    Inventors: Olivier SALLIN, Luc REYMOND, Kai Peter JOHNSSON
  • SEPIAPTERIN REDUCTASE INHIBITORS
    Publication number: 20170096435
    Abstract: Inhibitors of sepiapterin reductase and uses of sepiapterin reductase inhibitors in analgesia, treatment of acute and chronic pain, anti-inflammation, and immune cell regulation are disclosed.
    Type: Application
    Filed: September 30, 2016
    Publication date: April 6, 2017
    Inventors: Mark Joseph Tebbe, Holly Victoria Atton, Craig Avery, Steven Mark Bromidge, Mark Kerry, Adrian Kotei Kotey, Nathaniel J. Monck, Mirco Meniconi, Mark Peter Ridgill, Heather Tye, Eddine Saiah, Kai Peter Johnsson, Katarzyna Irena Gorska, Hairuo Peng, John Michael McCall
  • MEANS AND METHODS FOR BIOLUMINESCENCE RESONANCE ENERGY TRANSFER (BRET) ANALYSIS IN A BIOLOGICAL SAMPLE
    Publication number: 20160146794
    Abstract: The invention relates to the field of in vitro detection methods using luminescence. Provided is a sensor molecule for detecting an analyte of interest in a sample using bioluminescence resonance energy transfer (BRET), the sensor molecule comprising a proteinaceous moiety tethered to a synthetic regulatory molecule. Also provided is an analytical device comprising a sensor and methods using the sensor molecule.
    Type: Application
    Filed: July 18, 2013
    Publication date: May 26, 2016
    Inventors: Kai Peter Johnsson, Alberto Schena, Rudolf Griss