Abstract: A calibration device for surgical instruments includes a base, an instrument holder and at least one reference frame marker, the instrument holder is pivotally mounted on the base, and the reference frame marker is mounted on the base. The surgical instrument can be inserted into an insertion hole of the instrument holder to rotate or swing selectively on the base such that a signal processor of a surgical navigation system can receive coordinate signals from the reference frame marker and at least one coordinate marker mounted on the surgical instrument to calibrate the surgical instrument.