Abstract: A method for dual-SIM communication is applicable to an electronic device equipped with a physical SIM card. The method includes: activating the physical SIM card and successfully registering the physical SIM card with a network, and establishing a data channel for the physical SIM card; based on the data channel, acquiring a cloud SIM card assigned by a cloud SIM card server, and acquiring authentication response data acquired by initiating a registration authentication request by the cloud SIM card to a SIM card pool; and performing dual-SIM communication based on the physical SIM card and the cloud SIM card. According to the method, dual-SIM communication is implemented based on a primary-backup configuration of a physical SIM card and the cloud SIM card employed in the electronic device. The physical SIM card serves as both a data card and an authentication card for the cloud SIM card.

Abstract: Disclosed herein include methods, compositions, and kits suitable for use in polynucleotide assembly. Methods, compositions, systems, and kits provided herein can employ a strategy which implements highly specific external barcodes that are not incorporated into the final assembled product. In some embodiments, a highly specific DNA barcode pair forms an external third helix to hold synthetic fragments together at a temperature prohibiting interactions of short complementary toehold sequences alone before enzymatically ligating nicks in the lower strand to covalently fix the connection between fragments. The method can comprise removal of the external third helix either enzymatically, or by PCR amplification of the lower strand without the external third helix, to form a seamless connection.

Abstract: Disclosed herein include methods, compositions, and kits suitable for use in generation of genome-expanded organisms comprising, e.g., a chimeric genome. In some embodiments, the methods, systems and nucleic acid compositions include systems comprising a donor cell comprising one or more components of a delivery module and a recipient cell comprising one or more components of an integrator module. In some embodiments, the methods, systems and nucleic acid compositions comprise polynucleotides encoding one or more components of a delivery module, and one or more components of an integrator module.

Abstract: The provided are a cloud SIM allocation method and system, and a device and a storage medium thereof. The method includes: step S1, acquiring a camped cell identity (ID) and a timing advance (TA) value of a terminal device; step S2, determining a roaming state of the terminal device based on the camped cell ID and the TA value, the roaming state comprising an international roaming state and a local roaming state; and step S3, allocating a cloud SIM based on the roaming state of the terminal device. The camped Cell ID and TA value of the terminal device are utilized to identify a cross-border border state thereof. This avoids using signals from cross-border operators, and shields signal interference from neighboring countries. The cloud SIM allocation process is optimized, the time required for frequency band scanning is reduced, and the stability of network communication and user experience are enhanced.

Abstract: The provided are a method for switching a cloud SIM, and an electronic device and a computer-readable storage medium therefor. In the method, a first MCC is identified, whether the identified first MCC matches a second MCC is determined, and a new cloud SIM corresponding to a country or region of the first MCC is assigned to the electronic device in a case where the first MCC does not match the MCC. The country or region where the electronic device is located is determined using the MCC from the network identifier acquired during the connection process between the seed SIM and the cloud SIM server. This enables rapid and accurate identification of the cross-border movement of the electronic device and facilitates timely replacement of the cloud SIM in the electronic device. Consequently, while ensuring operational convenience, this method effectively improves use experience of the device and saves tariffs.

Abstract: Disclosed are a distributed management system for smart card management apparatuses and a management method therefor. The system includes: a service provision apparatus belonging to a service center, a plurality of smart card management apparatuses, and a plurality of terminals; wherein the service provision apparatus includes a service provision center and one or a plurality of proxy modules, and in the case that each of the plurality of terminals and a smart card management module are registered in the service provision center, the smart card management apparatus authenticates the terminal by a proxy module assigned by the service provision center, such that the terminal accesses a network servicing the terminal.

Abstract: A method for selecting a global roaming virtual subscriber identity module (SIM) card within a terminal, and a terminal therefor are provided. Within the terminal, a specific number of local virtual SIM cards from different operators are shared, that is, the bootstrap SIM cards. Prior to interaction between the terminal and a server based on a local virtual SIM card within the terminal after startup, the terminal checks whether registration record information of at least one local virtual SIM card within a region where the terminal resides is stored in the terminal; in the case that the registration record information is stored, the terminal selects a first local virtual SIM card that is successfully registered to interact with the server; and the terminal receives remote virtual SIM card information delivered by the server.

Abstract: Present invention relates to an apparatus and a method for establishing communication connections. The method includes: in response to a failure of a SIM card, acquiring first KI card information, establishing a first communication connection to a cloud SIM card management platform based on the first KI card information; acquiring cloud SIM card information from the cloud SIM card management platform over the first communication connection; and establishing a second communication connection based on the cloud SIM card information. In the case that the multi-SIM terminal device establishes the second communication connection based on the cloud SIM card information, the first communication connection may be disconnected, and the KI card information may be released. With the solutions, a success rate of establishing the first communication connection may be ensured on the premise of saving fees for users.

Abstract: The current invention provides a synthetic prokaryotic genome comprising 5 or fewer occurrences of one or more sense codons; and/or a synthetic prokaryotic genome derived from a parent genome, wherein the synthetic prokaryotic genome comprises less than 10%, 5%, 2%, 1%, 0.5%, 0.1% of the occurrences of one or more sense codons, relative to the parent genome; and/or a synthetic prokaryotic genome comprising 100 or more, 200 or more, or 1000 or more genes with no occurrences of one or more sense codons.

Abstract: The current invention provides a synthetic prokaryotic genome comprising 5 or fewer occurrences of one or more sense codons; and/or a synthetic prokaryotic genome derived from a parent genome, wherein the synthetic prokaryotic genome comprises less than 10%, 5%, 2%, 1%, 0.5%, 0.1% of the occurrences of one or more sense codons, relative to the parent genome; and/or a synthetic prokaryotic genome comprising 100 or more, 200 or more, or 1000 or more genes with no occurrences of one or more sense codons.

Abstract: Disclosed are a distributed management system for smart card management apparatuses and a management method therefor. The system includes: a service provision apparatus belonging to a service center, a plurality of smart card management apparatuses, and a plurality of terminals; wherein the service provision apparatus includes a service provision center and one or a plurality of proxy modules, and in the case that each of the plurality of terminals and a smart card management module are registered in the service provision center, the smart card management apparatus authenticates the terminal by a proxy module assigned by the service provision center, such that the terminal accesses a network servicing the terminal.

Abstract: The current invention provides a synthetic prokaryotic genome comprising 5 or fewer occurrences of one or more sense codons; and/or a synthetic prokaryotic genome derived from a parent genome, wherein the synthetic prokaryotic genome comprises less than 10%, 5%, 2%, 1%, 0.5%, 0.1% of the occurrences of one or more sense codons, relative to the parent genome; and/or a synthetic prokaryotic genome comprising 100 or more, 200 or more, or 1000 or more genes with no occurrences of one or more sense codons.

Abstract: A method includes: establishing a connection with a virtual SIM card server in a first network using a global virtual SIM card of a terminal to acquire a regional virtual SIM card from the virtual SIM card server, wherein the regional virtual SIM card is configured to allow the terminal to access a second network; determining whether the global virtual SIM card of the terminal is in conflict; updating a count of conflicts in response to determining that the global virtual SIM card of the terminal is in conflict; and upon elapse of a wait period, replacing the global virtual SIM card of the terminal with a new one to acquire the regional virtual SIM card, wherein the wait period is determined according to the count of conflicts and an identifier of the terminal.

Abstract: The current invention provides a synthetic prokaryotic genome comprising 5 or fewer occurrences of one or more sense codons; and/or a synthetic prokaryotic genome derived from a parent genome, wherein the synthetic prokaryotic genome comprises less than 10%, 5%, 2%, 1%, 0.5%, 0.1% of the occurrences of one or more sense codons, relative to the parent genome; and/or a synthetic prokaryotic genome comprising 100 or more, 200 or more, or 1000 or more genes with no occurrences of one or more sense codons.

Abstract: A method for handover between a private network and a public network based on cloud communications includes: receiving, by a cloud communication server, measurement information reported by a cloud communication terminal; determining, based on the measurement information and a network used by the cloud communication terminal, whether the cloud communication terminal satisfies a network handover condition; in response to determining that the cloud communication terminal satisfies the network handover condition, assigning a cloud SIM card to the cloud communication terminal, such that the cloud communication terminal establishes a data connection in a target network based on the cloud SIM card; and in response to receiving information indicative of successful establishment of the data connection in the target network from the cloud communication terminal, releasing a cloud SIM card used by the cloud communication terminal in a source network.

Abstract: A method and apparatus for sending subscriber identifiers are disclosed. The method includes: receiving information of a USIM card from a UICC in a cloud card pool over a first communication connection; receiving an identity request from a 5G SA network, wherein the identity request is for requesting acquiring a SUCI; acquiring the SUCI based on the identity request and the information of the USIM card; and sending the SUCI to the 5G SA network, wherein the SUCI is for establishing a second communication connection. The SUCI may be generated at the ME or at the UICC. The first communication connection may be a roaming communication connection and the second communication connection may be a non-roaming communication connection.

Abstract: The current invention provides a synthetic prokaryotic genome comprising 5 or fewer occurrences of one or more sense codons; and/or a synthetic prokaryotic genome derived from a parent genome, wherein the synthetic prokaryotic genome comprises less than 10%, 5%, 2%, 1%, 0.5%, 0.1% of the occurrences of one or more sense codons, relative to the parent genome; and/or a synthetic prokaryotic genome comprising 100 or more, 200 or more, or 1000 or more genes with no occurrences of one or more sense codons.

Abstract: Present invention relates to an apparatus and a method for establishing communication connections. The method includes: in response to a failure of a SIM card, acquiring first KI card information, establishing a first communication connection to a cloud SIM card management platform based on the first KI card information; acquiring cloud SIM card information from the cloud SIM card management platform over the first communication connection; and establishing a second communication connection based on the cloud SIM card information. In the case that the multi-SIM terminal device establishes the second communication connection based on the cloud SIM card information, the first communication connection may be disconnected, and the KI card information may be released. With the solutions, a success rate of establishing the first communication connection may be ensured on the premise of saving fees for users.

Abstract: The invention relates to a method comprising a) providing a host cell said host cell comprising an episomal replicon, said episomal replicon comprising a donor nucleic acid sequence, said host cell further comprising a target nucleic acid, b) providing helper protein(s) capable of supporting nucleic acid recombination in said host cell c) providing helper protein(s) and/or RNAs capable of supporting nucleic acid excision in said host cell wherein said donor nucleic acid sequence comprises in order. 5?-homologous recombination sequence 1-sequence of interest-homologous recombination sequence 2-3? wherein said sequence of interest comprises a positive selectable marker d) inducing excision of said donor nucleic acid sequence e) incubating to allow recombination between the excised donor nucleic acid and said target nucleic acid f) selecting for recombinants having incorporated said donor nucleic acid into said target nucleic acid. Also described are nucleic acids and cells.

Abstract: The current invention provides a synthetic prokaryotic genome comprising 5 or fewer occurrences of one or more sense codons; and/or a synthetic prokaryotic genome derived from a parent genome, wherein the synthetic prokaryotic genome comprises less than 10%, 5%, 2%, 1%, 0.5%, 0.1% of the occurrences of one or more sense codons, relative to the parent genome; and/or a synthetic prokaryotic genome comprising 100 or more, 200 or more, or 1000 or more genes with no occurrences of one or more sense codons.