Abstract: The current invention provides a synthetic prokaryotic genome comprising 5 or fewer occurrences of one or more sense codons; and/or a synthetic prokaryotic genome derived from a parent genome, wherein the synthetic prokaryotic genome comprises less than 10%, 5%, 2%, 1%, 0.5%, 0.1% of the occurrences of one or more sense codons, relative to the parent genome; and/or a synthetic prokaryotic genome comprising 100 or more, 200 or more, or 1000 or more genes with no occurrences of one or more sense codons.

Abstract: A method for handover between a private network and a public network based on cloud communications includes: receiving, by a cloud communication server, measurement information reported by a cloud communication terminal; determining, based on the measurement information and a network used by the cloud communication terminal, whether the cloud communication terminal satisfies a network handover condition; in response to determining that the cloud communication terminal satisfies the network handover condition, assigning a cloud SIM card to the cloud communication terminal, such that the cloud communication terminal establishes a data connection in a target network based on the cloud SIM card; and in response to receiving information indicative of successful establishment of the data connection in the target network from the cloud communication terminal, releasing a cloud SIM card used by the cloud communication terminal in a source network.

Abstract: A method and apparatus for sending subscriber identifiers are disclosed. The method includes: receiving information of a USIM card from a UICC in a cloud card pool over a first communication connection; receiving an identity request from a 5G SA network, wherein the identity request is for requesting acquiring a SUCI; acquiring the SUCI based on the identity request and the information of the USIM card; and sending the SUCI to the 5G SA network, wherein the SUCI is for establishing a second communication connection. The SUCI may be generated at the ME or at the UICC. The first communication connection may be a roaming communication connection and the second communication connection may be a non-roaming communication connection.

Abstract: The current invention provides a synthetic prokaryotic genome comprising 5 or fewer occurrences of one or more sense codons; and/or a synthetic prokaryotic genome derived from a parent genome, wherein the synthetic prokaryotic genome comprises less than 10%, 5%, 2%, 1%, 0.5%, 0.1% of the occurrences of one or more sense codons, relative to the parent genome; and/or a synthetic prokaryotic genome comprising 100 or more, 200 or more, or 1000 or more genes with no occurrences of one or more sense codons.

Abstract: Present invention relates to an apparatus and a method for establishing communication connections. The method includes: in response to a failure of a SIM card, acquiring first KI card information, establishing a first communication connection to a cloud SIM card management platform based on the first KI card information; acquiring cloud SIM card information from the cloud SIM card management platform over the first communication connection; and establishing a second communication connection based on the cloud SIM card information. In the case that the multi-SIM terminal device establishes the second communication connection based on the cloud SIM card information, the first communication connection may be disconnected, and the KI card information may be released. With the solutions, a success rate of establishing the first communication connection may be ensured on the premise of saving fees for users.

Abstract: The invention relates to a method comprising a) providing a host cell said host cell comprising an episomal replicon, said episomal replicon comprising a donor nucleic acid sequence, said host cell further comprising a target nucleic acid, b) providing helper protein(s) capable of supporting nucleic acid recombination in said host cell c) providing helper protein(s) and/or RNAs capable of supporting nucleic acid excision in said host cell wherein said donor nucleic acid sequence comprises in order. 5?-homologous recombination sequence 1-sequence of interest-homologous recombination sequence 2-3? wherein said sequence of interest comprises a positive selectable marker d) inducing excision of said donor nucleic acid sequence e) incubating to allow recombination between the excised donor nucleic acid and said target nucleic acid f) selecting for recombinants having incorporated said donor nucleic acid into said target nucleic acid. Also described are nucleic acids and cells.

Abstract: The current invention provides a synthetic prokaryotic genome comprising 5 or fewer occurrences of one or more sense codons; and/or a synthetic prokaryotic genome derived from a parent genome, wherein the synthetic prokaryotic genome comprises less than 10%, 5%, 2%, 1%, 0.5%, 0.1% of the occurrences of one or more sense codons, relative to the parent genome; and/or a synthetic prokaryotic genome comprising 100 or more, 200 or more, or 1000 or more genes with no occurrences of one or more sense codons.

Abstract: The invention relates to a method comprising a) providing a host cell said host cell comprising an episomal replicon, said episomal replicon comprising a donor nucleic acid sequence, said host cell further comprising a target nucleic acid, b) providing helper protein(s) capable of supporting nucleic acid recombination in said host cell c) providing helper protein(s) and/or RNAs capable of supporting nucleic acid excision in said host cell wherein said donor nucleic acid sequence comprises in order: 5?-homologous recombination sequence 1-sequence of interest-homologous recombination sequence 2-3? wherein said sequence of interest comprises a positive selectable marker d) inducing excision of said donor nucleic acid sequence e) incubating to allow recombination between the excised donor nucleic acid and said target nucleic acid f) selecting for recombinants having incorporated said donor nucleic acid into said target nucleic acid. Also described are nucleic acids and cells.

Abstract: Embodiments of the present disclosure disclose an access method, which comprises: establishing a first connection between a user equipment and a first mobile communication network according to a first subscriber identification number; requesting, via the first connection, a server to assign a subscriber identification number to the user equipment, wherein the subscriber identification number is a subscriber identification number corresponding to a SIM card in the server, and the subscriber identification number is a subscriber identification number at a location where the user equipment is currently located; acquiring, via the first connection, the second subscriber identification number assigned by the server; and establishing a second connection between the user equipment and a second mobile communication network servicing the current location according to the second subscriber identification number.

Abstract: Embodiments of the present invention provide a method and apparatus for authenticating a virtual subscriber identity module (VSIM). The method includes: establishing a first connection with a mobile communication network according to a removable subscriber identity module (RSIM); acquiring VSIM information sent by a VSIM server via the first connection, the VSIM information comprising a subscriber identification number; initiating a registration process to a network side according to the VSIM information; acquiring an authentication request, the authentication request carrying an authentication parameter to request for authentication for the VSIM; sending the authentication parameter to the VSIM server, such that the VSIM server determines an authentication response parameter according to the authentication parameter; and acquiring the authentication response parameter sent by the VSIM server, and performing authentication according to the authentication response parameter.

Abstract: Embodiments of the present invention provide a method and apparatus for authenticating a virtual subscriber identity module (VSIM). The method includes: establishing a first connection with a mobile communication network according to a removable subscriber identity module (RSIM); acquiring VSIM information sent by a VSIM server via the first connection, the VSIM information comprising a subscriber identification number; initiating a registration process to a network side according to the VSIM information; acquiring an authentication request, the authentication request carrying an authentication parameter to request for authentication for the VSIM; sending the authentication parameter to the VSIM server, such that the VSIM server determines an authentication response parameter according to the authentication parameter; and acquiring the authentication response parameter sent by the VSIM server, and performing authentication according to the authentication response parameter.

Abstract: Embodiments of the present disclosure disclose an access method, which comprises: establishing a first connection between a user equipment and a first mobile communication network according to a first subscriber identification number; requesting, via the first connection, a server to assign a subscriber identification number to the user equipment, wherein the subscriber identification number is a subscriber identification number corresponding to a SIM card in the server, and the subscriber identification number is a subscriber identification number at a location where the user equipment is currently located; acquiring, via the first connection, the second subscriber identification number assigned by the server; and establishing a second connection between the user equipment and a second mobile communication network servicing the current location according to the second subscriber identification number.

Abstract: There is provided a method for evolving an orthogonal rRNA molecule, comprising the steps of: providing one or more libraries of mutant orthogonal rRNA molecules and introducing the libraries into cells such that the orthogonal rRNA is incorporated into ribosomes to provide orthogonal ribosomes; providing one or more orthogonal mRNA molecules which (i) are not translated by natural ribosomes, and (ii) comprise one or more orthogonal mRNA codons; assaying the translation of the orthogonal mRNA and selecting the orthogonal rRNA molecules which translate the orthogonal mRNA, wherein the assay in step (c) requires translation of one or more orthogonal mRNA codons in the orthogonal mRNA; and orthogonal ribosomes incorporating such rRNA molecules.

Abstract: The invention relates to 16S rRNA comprising a mutation at A1196, and to 16S rRNA further comprising a mutation at C1195 and/or A1197, and to 16S rRNA which comprises (i) C1195A and A1196G; or (ii) C1195T, A1196G and A1197G; or (iii) A1196G and A1197G. The invention also relates to ribosomes comprising such 16S rRNAs and to use of same.

Abstract: There is provided a method for evolving an orthogonal rRNA molecule, comprising the steps of: providing one or more libraries of mutant orthogonal rRNA molecules and introducing the libraries into cells such that the orthogonal rRNA is incorporated into ribosomes to provide orthogonal ribosomes; providing one or more orthogonal mRNA molecules which (i) are not translated by natural ribosomes, and (ii) comprise one or more orthogonal mRNA codons; assaying the translation of the of the orthogonal mRNA and selecting the orthogonal rRNA molecules which translate the orthogonal mRNA, wherein the assay in step (c) requires translation of one or more orthogonal mRNA codons in the orthogonal mRNA; and orthogonal ribosomes incorporating such rRNA molecules.