Abstract: The present invention relates to a novel crosslinked polyethylenimine (PEI) nanoparticle based nucleic acid transfection agent wherein the crosslinker is having carbon chain in the range of C2 to C8, ranging between 3.27-19.8%, having the size of nanoparticle ranging between 20-600 nm and zeta potential ranging from +5 to 50 mV.

Abstract: Disclosed herein is a method for the preparation of oligonucleotide microarrays obviating the drawbacks to an extent, such as time consuming complex chemical reactions, preparation of modified supports/oligomer modifying reagents, use of activating/condensing reagent, low signal to noise ratio, poor immobilization and hybridization efficiencies, etc. Further, the prepared arrays can be used to detect single or multiple nucleotide mismatches using hybridization assay.

Abstract: The present invention relates to a novel crosslinked polyethylenimine (PEI) nanoparticle based nucleic acid transfection agent wherein the crosslinker is having carbon chain in the range of C2 to C8, ranging between 3.27-19.8%, having the size of nanoparticle ranging between 20-600 nm and zeta potential ranging from +5 to 50 mV.

Abstract: The present invention provides a reusable, universal polymer support for repetitive oligonucleotide synthesis and a method of synthesis thereof.

Abstract: The present invention lies in developing a novel method for the preparation of oligonucleotide microarrays obviating the drawbacks to an extent, such as time consuming complex chemical reactions, preparation of modified supports/oligomer modifying reagents, use of activating/condensing reagent, low signal to noise ratio, poor immobilization and hybridization efficiencies, etc. Further, the prepared arrays can be used to detect single or multiple nucleotide mismatches using hybridization assay.

Abstract: A universal polymer support containing an organic aliphatic molecule of structure ##STR1## having at least a pair of cis-hydroxyl groups where one of the hydroxyl groups is attached to the polymer support through a covalent linkage and the other hydroxyl group is protected by an acid labile group.

Abstract: A process for preparing oligoribo- or oligodeoxyribonucleotides comprising treating an alkanediol or alkanetriol of formula I ##STR1## wherein R.sup.1 =H--(CH.sub.2).sub.n --; andR.sup.2 =--CH.sub.2 OH or --(CH.sub.2).sub.n --Hn=1-4;with 4,4'-dimethoxytrityl chloride to generate a monosubstituted tritylated compound of formula II ##STR2## wherein R.sup.3 =H--(CH.sub.2).sub.p --; andR.sup.4 =-CH.sub.2 OH or --(CH.sub.2).sub.p --H;R.sup.5 is 4,4'-dimethoxytrityl and concommitantly R.sup.6 is hydrogen, orR.sup.5 is hydrogen and concommitantly R.sup.6 is 4,4'-dimethoxytrityl;p=1-4;and treating the compound of formula II with one equivalent of a homobifunctional alkanedioic acid halide, and contacting the resulting mixture with a polymer support bearing hydroxyl or aminoalkyl functionalities.