Patents by Inventor Kaile Wang

Kaile Wang has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Sequencing library, and preparation and use thereof
    Patent number: 11702690
    Abstract: The present invention discloses a sequencing library comprising a nucleotide sequence. The sequence comprises a linker sequence and two target sequences. Two ends of the linker sequence are respectively linked to the target sequences and the two target sequences are direct repeat sequences. The present invention further discloses preparation and use of the sequencing library. The present invention overcomes the high error rate problem of current DNA sequencing technologies, especially in a way of very low coverage bias, and can be used to detect low frequency mutations in different kinds of samples.
    Type: Grant
    Filed: February 23, 2018
    Date of Patent: July 18, 2023
    Assignee: Agricultural Genomics Institute at Shenzhen China Academy of Agricultural Sciences
    Inventors: Jue Ruan, Kaile Wang
  • METHODS AND APPLICATIONS FOR CELL BARCODING
    Publication number: 20220205035
    Abstract: The current methods and compositions of the disclosure provide a platform for detecting the transcriptomic, genomic, or proteomic profile in relation to particular characteristics of a single cell, such as the location of a cell within a tissus. Accordingly, aspects of the disclosure relate to a method for barcoding eukaryotic cell nuclei comprising: transferring oligonucleotides into the nuclei of cells and performing single-cell analysis to identify the sequence of the barcode; wherein the oligonucleotides comprise a barcode region and a target region.
    Type: Application
    Filed: April 3, 2020
    Publication date: June 30, 2022
    Applicant: Board of Regents, The University of Texas System
    Inventors: Nicholas E. NAVIN, Kaile WANG
  • Sequencing library, preparation method and use thereof
    Patent number: 10718015
    Abstract: The present invention provides a sequencing library, and the sequencing library has an inserted fragment which is an equidirectional alternating concatemer of a sequence to be tested and a tag sequence. The present invention further provides a method for preparing the sequencing library. The present invention also provides a sequencing method. The sequencing library and sequencing method as provided in the present invention are capable of removing DNA amplification errors and sequencing errors under any sequencing depths, so that mutations of DNA molecules could be ultra-accurately determined. The sequencing library of the present invention is suitable for construction of a sequencing library of trace short DNA fragments and even of single-strand DNAs.
    Type: Grant
    Filed: December 5, 2014
    Date of Patent: July 21, 2020
    Assignee: Beijing Institute of Genomica, Chinese Academy of Sciences
    Inventors: Jue Ruan, Kaile Wang, Chung-I Wu, Xuemei Lu
  • SEQUENCING LIBRARY, AND PREPARATION AND USE THEREOF
    Publication number: 20190078157
    Abstract: The present invention discloses a sequencing library comprising a nucleotide sequence. The sequence comprises a linker sequence and two target sequences. Two ends of the linker sequence are respectively linked to the target sequences and the two target sequences are direct repeat sequences. The present invention further discloses preparation and use of the sequencing library. The present invention overcomes the high error rate problem of current DNA sequencing technologies, especially in a way of very low coverage bias, and can be used to detect low frequency mutations in different kinds of samples.
    Type: Application
    Filed: February 23, 2018
    Publication date: March 14, 2019
    Inventors: Jue Ruan, Kaile Wang
  • SEQUENCING LIBRARY, PREPARATION METHOD AND USE THEREOF
    Publication number: 20160362735
    Abstract: The present invention provides a sequencing library, and the sequencing library has an inserted fragment which is an equidirectional alternating concatemer of a sequence to be tested and a tag sequence. The present invention further provides a method for preparing the sequencing library. The present invention also provides a sequencing method. The sequencing library and sequencing method as provided in the present invention are capable of removing DNA amplification errors and sequencing errors under any sequencing depths, so that mutations of DNA molecules could be ultra-accurately determined. The sequencing library of the present invention is suitable for construction of a sequencing library of trace short DNA fragments and even of single-strand DNAs.
    Type: Application
    Filed: December 5, 2014
    Publication date: December 15, 2016
    Applicant: Beijing Institute of Genomics, Chinese Academy of Science
    Inventors: Jue Ruan, Kaile Wang, Chung-I Wu, Xuemei Lu