Abstract: The present invention discloses a sequencing library comprising a nucleotide sequence. The sequence comprises a linker sequence and two target sequences. Two ends of the linker sequence are respectively linked to the target sequences and the two target sequences are direct repeat sequences. The present invention further discloses preparation and use of the sequencing library. The present invention overcomes the high error rate problem of current DNA sequencing technologies, especially in a way of very low coverage bias, and can be used to detect low frequency mutations in different kinds of samples.

Abstract: The current methods and compositions of the disclosure provide a platform for detecting the transcriptomic, genomic, or proteomic profile in relation to particular characteristics of a single cell, such as the location of a cell within a tissus. Accordingly, aspects of the disclosure relate to a method for barcoding eukaryotic cell nuclei comprising: transferring oligonucleotides into the nuclei of cells and performing single-cell analysis to identify the sequence of the barcode; wherein the oligonucleotides comprise a barcode region and a target region.

Abstract: A system and method for content selection and presentation is disclosed. A plurality of content elements configured for presentation in at least one content container is received and one of the plurality of content elements is selected for presentation in the at least one content container. The one of the plurality of content elements is selected by a trained selection model based on an optimal impression allocation. An interface is generated that includes the selected one of the plurality of content elements.

Abstract: A system and method for content selection and presentation is disclosed. A plurality of content elements configured for presentation in at least one content container is received and one of the plurality of content elements is selected for presentation in the at least one content container. The one of the plurality of content elements is selected by a trained selection model based on an optimal impression allocation. An interface is generated that includes the selected one of the plurality of content elements.

Abstract: The present invention provides a sequencing library, and the sequencing library has an inserted fragment which is an equidirectional alternating concatemer of a sequence to be tested and a tag sequence. The present invention further provides a method for preparing the sequencing library. The present invention also provides a sequencing method. The sequencing library and sequencing method as provided in the present invention are capable of removing DNA amplification errors and sequencing errors under any sequencing depths, so that mutations of DNA molecules could be ultra-accurately determined. The sequencing library of the present invention is suitable for construction of a sequencing library of trace short DNA fragments and even of single-strand DNAs.

Abstract: The present invention discloses a sequencing library comprising a nucleotide sequence. The sequence comprises a linker sequence and two target sequences. Two ends of the linker sequence are respectively linked to the target sequences and the two target sequences are direct repeat sequences. The present invention further discloses preparation and use of the sequencing library. The present invention overcomes the high error rate problem of current DNA sequencing technologies, especially in a way of very low coverage bias, and can be used to detect low frequency mutations in different kinds of samples.

Abstract: The present invention provides high potency 3,3?-diindolylmethane (DIM) and analogue compounds as antifouling agents. The present invention further provides methods of preventing or reducing settlement and/or fouling of marine fouling organisms on surfaces of submerged objects by coating the surfaces with the 3,3?-diindolylmethane (DIM) and analogue compounds provided.

Abstract: The present invention provides a sequencing library, and the sequencing library has an inserted fragment which is an equidirectional alternating concatemer of a sequence to be tested and a tag sequence. The present invention further provides a method for preparing the sequencing library. The present invention also provides a sequencing method. The sequencing library and sequencing method as provided in the present invention are capable of removing DNA amplification errors and sequencing errors under any sequencing depths, so that mutations of DNA molecules could be ultra-accurately determined. The sequencing library of the present invention is suitable for construction of a sequencing library of trace short DNA fragments and even of single-strand DNAs.

Abstract: The present invention provides high potency 3,3?-diindolylmethane (DIM) and analogue compounds as antifouling agents. The present invention further provides methods of preventing or reducing settlement and/or fouling of marine fouling organisms on surfaces of submerged objects by coating the surfaces with the 3,3?-diindolylmethane (DIM) and analogue compounds provided.

Abstract: The claims provide for detecting prions in a sample by (a) subjecting a sample to membrane-based electrophoresis to separate and/or concentrate at least some prions present in the sample; and (b) detecting and or identifying the presence of the separated and/or concentrated prions.

Abstract: Methods for removing a non-enveloped virus from a sample containing a target compound and non-enveloped virus contamination place the sample containing the target compound in a solvent chamber of an electrophoresis apparatus having a cathode and an anode, and a first solvent chamber being separated from a second solvent chamber by a separation barrier that has a defined pore size. The solvent chambers and the separation barrier are located between the cathode and anode.

Abstract: A method for recovering a desired virus type from a mixture of unwanted compounds places a mixture in a first sample chamber of an electrophoresis apparatus that contains a separation membrane located between the first sample chamber and a second sample chamber. Applying an electric potential across the first and second sample chambers separates at least a portion of one virus type on one side of the separation membrane while unwanted compounds are located on the other side of the separation membrane. Either the desired virus type or the unwanted molecules move through the separation membrane. The potential is applied until the required amount of desired virus type is located on one side of the separation membrane. Approximately 50% or more of the desired virus type that is located on one side of the separation membrane remains viable or substantially unchanged after recovery.

Abstract: The claims relate to methods and assays for capturing or detecting prions in a sample using prion-binding materials as well as methods of separating prions from a sample. A method selects a prion-binding material in the form of fibrin(ogen), fibrin(ogen)-related material, fibrin(ogen)-derived material, or mixtures thereof, and contacts a sample with the prion-binding material, such that prions contained in the sample are bound to or associated with the prion-binding material. In another aspect, an assay for detecting the presence of prions obtains prion-binding material in the form of fibrin(ogen), a fibrin(ogen)-related material, a fibrin(ogen)-derived material, or mixtures thereof, contacts the prion-binding material with the sample which may contain prions such that prions contained in the sample are bount to or associated with the prion-binding material, and tests for the presence of prions associated with or bound to the prion-binding material.

Abstract: The claims provide for detecting prions in a sample by (a) subjecting a sample to membrane-based electrophoresis to separate and/or concentrate at least some prions present in the sample; and (b) detecting and or identifying the presence of the separated and/or concentrated prions.