Abstract: The present invention relates to a formulation comprising an anti-IL-23p19 antibody, and in particular to a pharmaceutical formulation comprising an anti-IL-23p19 antibody, a buffer, a stabilizer and a surfactant. Furthermore, the present invention further relates to therapeutic or prophylactic use of these formulations.

Abstract: The present invention relates to formulations comprising an anti-PD-1/HER2 bispecific antibody, and in particular to a pharmaceutical formulation comprising the anti-PD-1/HER2 bispecific antibody, a buffer, a stabilizer and a surfactant. Furthermore, the present invention also relates to therapeutic or prophylactic use of these formulations.

Abstract: The present invention discloses a cell culture method based on high-density and continuous inoculation and use thereof. The method comprises the following steps: (1) providing a cell culture, and performing resuscitation, shake flask amplification culture and rocking reaction bag amplification culture on the cell culture; (2) transferring the resuscitated and amplified cells into a last-stage cell amplification tank for continuous amplification culture; (3) inoculating the cells in the last-stage cell amplification tank into a culture fermentation tank in a high-density and continuous inoculation mode for fermentation culture; and (4) harvesting a target product. The cell culture method based on high-density and continuous inoculation provided herein can reduce the average cell passage time of each batch and improve the production efficiency of cells and expression products thereof.

Abstract: The present invention relates to formulations comprising an anti-CD47/PD-L1 bispecific antibody, and in particular to pharmaceutical formulations comprising an anti-CD47/PD-L1 bispecific antibody, a buffer, a stabilizer and a surfactant. Furthermore, the present invention also relates to therapeutic or prophylactic use of these formulations.

Abstract: The present invention relates to formulations comprising an anti-LAG-3 antibody, and in particular to pharmaceutical formulations comprising an antibody specifically binding to LAG-3 molecules, a buffer, a stabilizer and a surfactant. Furthermore, the present invention also relates to therapeutic or prophylactic use of these formulations.

Abstract: The present invention relates to a formulation comprising an anti-CD47 antibody, and in particular to a pharmaceutical formulation comprising an antibody, a buffer, a stabilizer and a surfactant. Furthermore, the present invention also relates to therapeutic or prophylactic use of those formulations.

Abstract: The invention provides methods of producing functional split inteins having small N-intein and/or small C-intein. Using these split inteins with their protein trans-splicing and cleavage activities, we provide new and more effective methods of manipulating proteins. They include site-specific addition of synthetic peptides at protein's terminal and internal locations, ligation of synthetic and/or expressed polypeptides, controllable cyclization of synthetic and/or expressed polypeptides, and controllable cleavages of recombinant proteins. These methods have numerous utilities including but not limited to protein fluorescence labeling, fixation on microchips, site-specific PEGylation, and linkage with pharmaceutical molecules.

Abstract: The invention provides methods of producing functional split inteins having small N-intein and/or small C-intein. Using these split inteins with their protein trans-splicing and cleavage activities, we provide new and more effective methods of manipulating proteins. They include site-specific addition of synthetic peptides at protein's terminal and internal locations, ligation of synthetic and/or expressed polypeptides, controllable cyclization of synthetic and/or expressed polypeptides, and controllable cleavages of recombinant proteins. These methods have numerous utilities including but not limited to protein fluorescence labeling, fixation on microchips, site-specific PEGylation, and linkage with pharmaceutical molecules.

Abstract: The present invention relates to a method of preparing modified polypeptides, by linking a target polypeptide to a carrier molecule that is designed to bear one or more water-soluble polymer molecules, via protein trans-splicing. The polymer molecules can be attached to the carrier molecule either before or after ligation to the target polypeptide. Novel protein trans-splicing elements (known as “split inteins”) and trans-splicing partners are also provided.