Patents by Inventor Kalyan Handique

Kalyan Handique has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • MICROFLUIDIC SYSTEM FOR AMPLIFYING AND DETECTING POLYNUCLEOTIDES IN PARALLEL
    Publication number: 20210139951
    Abstract: The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.
    Type: Application
    Filed: January 11, 2021
    Publication date: May 13, 2021
    Inventors: Kalyan Handique, Sundaresh N. Brahmasandra, Karthik Ganesan, Jeff Williams
  • MOVING MICRODROPLETS IN A MICROFLUIDIC DEVICE
    Publication number: 20210123090
    Abstract: The present invention relates to a system and method for moving samples, such as fluid, within a microfluidic system using a plurality of gas actuators for applying pressure at different locations within the microfluidic. The system includes a substrate which forms a fluid network through which fluid flows, and a plurality of gas actuators integral with the substrate. One such gas actuator is coupled to the network at a first location for providing gas pressure to move a microfluidic sample within the network. Another gas actuator is coupled to the network at a second location for providing gas pressure to further move at least a portion of the microfluidic sample within the network. A valve is coupled to the microfluidic network so that, when the valve is closed, it substantially isolates the second gas actuator from the first gas actuator.
    Type: Application
    Filed: September 24, 2020
    Publication date: April 29, 2021
    Inventors: Kalyan Handique, Gene Parunak
  • HEATER UNIT FOR MICROFLUIDIC DIAGNOSTIC SYSTEM
    Publication number: 20210121887
    Abstract: The present technology provides for a heater substrate that contains networks of heater elements configured to controllably and selectively deliver heat to one or more PCR reaction chambers in a microfluidic substrate with which the heater substrate makes contact. In exemplary embodiments, the heater substrate can deliver heat to 12, 24, 48, or 96 chambers independently of one another, or simultaneously. The heater substrate is located in a heater unit that may be introduced into a diagnostic apparatus that can receive and position a microfluidic substrate, such as in a cartridge, in contact with the heater unit, receive one or more polynucleotide containing samples into one or more lanes in the microfluidic substrate, and cause amplification of the polynucleotides to occur, and detect presence of absence of specified polynucleotides in the amplified samples.
    Type: Application
    Filed: July 9, 2020
    Publication date: April 29, 2021
    Inventor: Kalyan Handique
  • System and method for capturing and analyzing cells
    Patent number: 10975422
    Abstract: A system and method for capturing and analyzing a set of cells, comprising: an array including a set of parallel pores, each pore including a chamber including a chamber inlet and a chamber outlet, and configured to hold a single cell, and a pore channel fluidly connected to the chamber outlet; an inlet channel fluidly connected to each chamber inlet of the set of parallel pores; an outlet channel fluidly connected to each pore channel of the set of parallel pores; a set of electrophoresis channels fluidly coupled to the outlet channel, configured to receive a sieving matrix for electrophoretic separation; and a set of electrodes including a first electrode and a second electrode, wherein the set of electrodes is configured to provide an electric field that facilitates electrophoretic analysis of the set of cells.
    Type: Grant
    Filed: July 30, 2018
    Date of Patent: April 13, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Kalyan Handique, Priyadarshini Gogoi, Saedeh Sepehri Javdani, Yi Zhou
  • PROCESSING PARTICLE-CONTAINING SAMPLES
    Publication number: 20210087609
    Abstract: A microfluidic device includes an input port for inputting a particle-containing liquidic samples into the device, a retention member, and a pressure actuator. The retention member is in communication with the input port and is configured to spatially separate particles of the particle-containing liquidic sample from a first portion of the liquid of the particle containing fluidic sample. The pressure actuator recombines at least some of the separated particles with a subset of the first portion of the liquid separated from the particles. The device can also include a lysing chamber that receives the particles and liquid from the retention member. The lysing chamber thermally lyses the particles to release contents thereof.
    Type: Application
    Filed: December 7, 2020
    Publication date: March 25, 2021
    Inventors: Kalyan Handique, Gene Parunak, Aaron Kehrer, Betty Wu, Karthik Ganesan
  • System and method for leakage control in a particle capture system
    Patent number: 10947581
    Abstract: A system and method for target material capture, the method comprising: receiving a set of target cells into an array of wells defined at a surface plane of a substrate; receiving a set of particles into the array of wells, thereby co-capturing the set of target cells and the set of particles; achieving a desired state for the array of wells upon receiving a washing fluid into a cavity in communication with the array of wells; receiving a lysis buffer into the cavity; receiving a partitioning fluid into the cavity, thereby displacing the lysis buffer from the cavity and partitioning each of the array of wells from adjacent wells, at the surface plane; and retaining intercellular material of the set of target cells, individually with the set of particles within the array of wells.
    Type: Grant
    Filed: March 17, 2020
    Date of Patent: March 16, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Kalyan Handique, John Stark, Yadwinder Deol, Vishal Sharma
  • SCANNING REAL-TIME MICROFLUIDIC THERMOCYCLER AND METHODS FOR SYNCHRONIZED THERMOCYCLING AND SCANNING OPTICAL DETECTION
    Publication number: 20210071234
    Abstract: Systems and methods for performing simultaneous nucleic acid amplification and detection. The systems and methods comprise methods for managing a plurality of protocols in conjunction with directing a sensor array across each of a plurality of reaction chambers. In certain embodiments, the protocols comprise thermocycling profiles and the methods may introduce offsets and duration extensions into the thermocycling profiles to achieve more efficient detection behavior.
    Type: Application
    Filed: September 21, 2020
    Publication date: March 11, 2021
    Inventors: Thomas Catalino Gubatayao, Kalyan Handique, Karthik Ganesan, Daniel M. Drummond
  • FLUORESCENCE DETECTOR FOR MICROFLUIDIC DIAGNOSTIC SYSTEM
    Publication number: 20210060565
    Abstract: The present technology provides for a fluorescent detector that is configured to detect light emitted for a probe characteristic of a polynucleotide. The polynucleotide is undergoing amplification in a microfluidic channel with which the detector is in optical communication. The detector is configured to detect minute quantities of polynucleotide, such as would be contained in a microfluidic volume. The detector can also be multiplexed to permit multiple concurrent measurements on multiple polynucleotides concurrently.
    Type: Application
    Filed: October 30, 2020
    Publication date: March 4, 2021
    Inventors: Kalyan Handique, Sundaresh N. Brahmasandra
  • METHOD FOR PROCESSING POLYNUCLEOTIDE-CONTAINING SAMPLES
    Publication number: 20210047676
    Abstract: Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.
    Type: Application
    Filed: March 27, 2020
    Publication date: February 18, 2021
    Inventors: Betty Wu, John S. Althaus, Nikhil Phadke, Sundaresh N. Brahmasandra, Kalyan Handique, Aaron Kehrer, Gene Parunak, Cecelia Haley, Ted Springer
  • Cell capture system and method of use
    Patent number: 10921237
    Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.
    Type: Grant
    Filed: August 28, 2020
    Date of Patent: February 16, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Kalyan Handique
  • SYSTEM AND METHOD FOR ISOLATING AND ANALYZING CELLS
    Publication number: 20210040567
    Abstract: A system and method for isolating cells, comprising: a substrate having a broad surface; an array comprising a set of wells defined at the broad surface of the substrate, each well including: a base surface, an open surface directly opposing the base surface, defined at the broad surface of the substrate, and configured to receive one of a single cell and a single cluster of cells from a direction perpendicular to the broad surface of the substrate, and a set of channels that fluidly couple each well to at least one adjacent well; wherein the set of wells includes an interior subset and an exterior subset fluidly coupled to and surrounding the interior subset by way of the set of channels; and a fluid delivery module surrounding the array and fluidly coupled to each well in the set of wells.
    Type: Application
    Filed: October 22, 2020
    Publication date: February 11, 2021
    Inventors: Kalyan Handique, Priyadarshini Gogoi, Yi Zhou, Saedeh Sepehri, Christopher Siemer
  • System and method for retrieving and analyzing particles
    Patent number: 10914672
    Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.
    Type: Grant
    Filed: September 18, 2020
    Date of Patent: February 9, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Kalyan Handique, Austin Payne, Vishal Sharma, Kyle Gleason, Priyadarshini Gogoi, Karthik Ganesan, Brian Boniface, Will Chow
  • Integrated system for processing microfluidic samples, and method of using the same
    Patent number: 10913061
    Abstract: This patent application describes an integrated apparatus for processing polynucleotide-containing samples, and for providing a diagnostic result thereon. The apparatus is configured to receive a microfluidic cartridge that contains reagents and a network for processing a sample. Also described are methods of using the apparatus.
    Type: Grant
    Filed: December 30, 2019
    Date of Patent: February 9, 2021
    Assignee: HANDYLAB, INC.
    Inventors: Kalyan Handique, Sundaresh N. Brahmasandra, Karthik Ganesan, Betty Wu, Nikhil Phadke, Gene Parunak, Jeff Williams
  • System and method for automated single cell processing
    Patent number: 10900032
    Abstract: A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements; a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including mRNA capture, cDNA synthesis, protein-associated assays, and library preparation, for next generation sequencing.
    Type: Grant
    Filed: May 5, 2020
    Date of Patent: January 26, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Kalyan Handique, Austin Payne, Sida Wang, Patrick Michael Tegels, William T. Koederitz, Daniel Genord, Grey Parker, Brian Boniface, Katlyn Curtin Mehne, Alec William Hitchiner
  • Microfluidic system for amplifying and detecting polynucleotides in parallel
    Patent number: 10900066
    Abstract: The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.
    Type: Grant
    Filed: June 26, 2020
    Date of Patent: January 26, 2021
    Assignee: HandyLab, Inc.
    Inventors: Kalyan Handique, Sundaresh N. Brahmasandra, Karthik Ganesan, Jeff Williams
  • MICROFLUIDIC SYSTEM FOR AMPLIFYING AND DETECTING POLYNUCLEOTIDES IN PARALLEL
    Publication number: 20210010059
    Abstract: The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.
    Type: Application
    Filed: February 11, 2020
    Publication date: January 14, 2021
    Inventors: Kalyan Handique, Sundaresh N. Brahmasandra, Karthik Ganesan, Jeff Williams
  • MICROFLUIDIC VALVE AND METHOD OF MAKING SAME
    Publication number: 20210001334
    Abstract: The present technology provides for a microfluidic substrate configured to carry out PCR on a number of polynucleotide-containing samples in parallel. The substrate can be a single-layer substrate in a microfluidic cartridge. Also provided are a method of making a microfluidic cartridge comprising such a substrate. Still further disclosed are a microfluidic valve suitable for use in isolating a PCR chamber in a microfluidic substrate, and a method of making such a valve.
    Type: Application
    Filed: July 10, 2020
    Publication date: January 7, 2021
    Inventors: Kalyan Handique, Gene Parunak, Cecelia Haley
  • SYSTEM AND METHOD FOR RETRIEVING AND ANALYZING PARTICLES
    Publication number: 20210003496
    Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.
    Type: Application
    Filed: September 18, 2020
    Publication date: January 7, 2021
    Inventors: Kalyan Handique, Austin Payne, Vishal Sharma, Kyle Gleason, Priyadarshini Gogoi, Karthik Ganesan, Brian Boniface, Will Chow
  • Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
    Patent number: 10875022
    Abstract: The technology described herein generally relates to systems for extracting polynucleotides from multiple samples, particularly from biological samples, and additionally to systems that subsequently amplify and detect the extracted polynucleotides. The technology more particularly relates to microfluidic systems that carry out PCR on multiple samples of nucleotides of interest within microfluidic channels, and detect those nucleotides.
    Type: Grant
    Filed: September 7, 2018
    Date of Patent: December 29, 2020
    Assignee: HANDYLAB, INC.
    Inventors: Jeff Williams, Kerry Wilson, Kalyan Handique
  • CELL CAPTURE SYSTEM AND METHOD OF USE
    Publication number: 20200393358
    Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.
    Type: Application
    Filed: August 28, 2020
    Publication date: December 17, 2020
    Inventor: Kalyan Handique