Patents by Inventor Kan-Chien LI

Kan-Chien LI has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method of SNP detection by using gene detection technique in bead-based microfluidics
    Patent number: 9080202
    Abstract: The present invention provides a method of SNP detection by using gene detection in bead-based microfluidics comprising following steps: (a) preparing a microbead with a duplex DNA; (b) inserting a dye into the duplex DNA; (c) delivering the microbead into a microchannel with a temperature gradient region; (d) heating the temperature gradient region to denature the duplex DNA; (e) monitoring a fluorescence intensity of the duplex DNA during the step (d) to obtain a melting curve; and (f) determining the SNP by a melting curve analysis method; wherein the duplex DNA is synthesized by a target single-strand DNA and an allele-specific probe. Furthermore, the present invention offers a temperature gradient region to enhance the measurement accuracy. The continuous-flow mechanism in this region is validated and optimized.
    Type: Grant
    Filed: January 17, 2014
    Date of Patent: July 14, 2015
    Assignee: NATIONAL TAIWAN UNIVERSITY
    Inventors: Kan-Chien Li, Shih-Torng Ding, En-Chung Lin, Yen-Wen Lu
  • METHOD OF SNP DETECTION BY USING GENE DETECTION TECHNIQUE IN BEAD-BASED MICROFLUIDICS
    Publication number: 20150125855
    Abstract: The present invention provides a method of SNP detection by using gene detection in bead-based microfluidics comprising following steps: (a) preparing a microbead with a duplex DNA; (b) inserting a dye into the duplex DNA; (c) delivering the microbead into a microchannel with a temperature gradient region; (d) heating the temperature gradient region to denature the duplex DNA; (e) monitoring a fluorescence intensity of the duplex DNA during the step (d) to obtain a melting curve; and (f) determining the SNP by a melting curve analysis method; wherein the duplex DNA is synthesized by a target single-strand DNA and an allele-specific probe. Furthermore, the present invention offers a temperature gradient region to enhance the measurement accuracy. The continuous-flow mechanism in this region is validated and optimized.
    Type: Application
    Filed: January 17, 2014
    Publication date: May 7, 2015
    Applicant: National Taiwan University
    Inventors: Kan-Chien LI, Shih-Torng DING, En-Chung LIN, Yen-Wen LU