Abstract: Systems and methods for screening enzyme variants are described, wherein the enzyme variants may catalyze a reaction that is cofactor-dependent or cofactor-independent.

Abstract: A transaxle of a rail vehicle includes: a power assembly; an axle body; a running wheel; a guiding frame; a horizontal wheel; and a connecting rod component, including a first transverse pull rod and a second transverse pull rod, where when the rail vehicle turns left, the horizontal wheel cooperates with a rail beam to drive the guiding frame to swing and drive the first transverse pull rod to move together, and the second transverse pull rod is driven by the first transverse pull rod to drive the running wheel to swing to the left, and when the rail vehicle turns right, the horizontal wheel cooperates with the rail beam to drive the guiding frame to swing and drive the first transverse pull rod to move together, and the second transverse pull rod is driven by the first transverse pull rod to drive the running wheel to swing to the right.

Abstract: An intelligent conference projection system includes a on-site master control system, and a conference system and an auxiliary system connected with the on-site master control system by signal connection. The conference system has a conference initiation module, a video communication module and an automatic incoming call projection awaking module. The auxiliary system includes a wireless transmission screen subsystem, a voice receiving and recognizing subsystem and a dual-mode touchpad subsystem.

Abstract: An LED filament and lamp, and a manufacturing process of an LED filament, the LED filament includes a PCB substrate and multiple LED chips, connecting circuits are provided on the PCB substrate, a positive pin and a negative pin are provided at two ends of the connecting circuit, respectively, and each LED chip is electrically connected to the connecting circuit; and a fluorescent glue layer is provided outside the PCB substrate and the LED chips, and the positive pin and the negative pin are exposed from the fluorescent glue layer. The LED lamp includes the LED filament. The manufacturing process of an LED filament enables manufacturing an LED filament. For the LED filament and lamp, and the manufacturing process of an LED filament, a PCB substrate is used as a base material, connecting circuits are fabricated on the PCB substrate, the connecting circuits are directly used for connecting LED chips.

Abstract: A geothermal energy system utilizes supercritical CO2 turbine and a radial outflow reaction turbine, a Catherine Wheel having wheel arms, that spins around an axle to produce power. A fin portion extends from the radial portion at an offset angle, to an exhaust end. A first working fluid, such as supercritical carbon dioxide flows through an arm conduit within the wheel arm and a second working fluid, such as a hydrocarbon mixes with the first working fluid and both flow through a turbine. The turbine may be configured within the wheel arm conduit or mounted prior to the Catherine Wheel or any other radial outflow reaction turbine, or variable phase turbines available, and it turns as the combined working fluids expand and vaporize. The second working fluid may be condensed and recirculated while the first working fluid is expelled back into a geothermal reservoir.

Abstract: A smart Internet high-definition scanner with laser correction is provided. The scanner may include a calibration laser emission unit located above a scanning area, an image collection unit and an image processing unit. This scanner may collect an image of a page of an unfolded book by using a camera (image collection unit) and a plurality of laser beams directed toward an open book from the calibration laser emission unit.

Abstract: Provided is a method for flattening a laser-based image of a curved book page, which is applicable to a scanning platform and an image acquisition device and a laser generation device fixed above the scanning platform. The flattening method includes the following steps of: opening a book on a horizontally arranged scanning platform, and emitting a laser beam to a curved book page to be scanned, the laser beam forming a plurality of laser lines parallel on the curved book page; acquiring a horizontal image of the curved book page having the parallel laser lines, and acquiring a 2D planar coordinate (x, y) of each pixel on the laser line in the horizontal image; calculating, according a y-coordinate in the 2D planar coordinate, a y-coordinate of an intersection line of the laser beam and the scanning platform, and an intersection angle of the laser beam and the scanning platform, and by means of a tangent function, a height of each pixel on the laser line, i.e.

Abstract: The application relates to the technical field of oil and gas field development, and discloses a correction method and system for a permeability model considering gas hydrate distribution, and a method and system for determining the permeability of the hydrate-bearing porous medium.

Abstract: A geothermal energy system utilizes a Catherine Wheel that spins around an axle to produce power. The Catherine Wheel has a plurality of wheel arms extending radially out from the axle. A fin portion extends from the radial portion at an offset angle, to an exhaust end. A first working fluid, such as supercritical carbon dioxide flows through an arm conduit within the wheel arm and second working fluid, such as a simply hydrocarbon mixes with the first working fluid and both flow through a turbine. The turbine is configured within the wheel arm conduit and it turns as the combined working fluids vaporize. The fluids are expelled and the second working fluid may be condensed and recirculated while the first working fluid may be expelled back into a geothermal reservoir. The geothermal energy system efficiently and effectively produces power by using geothermal energy to vaporize a hydrocarbon.

Abstract: A marker for occluding a foreign matter in an acquired image contains a mark part whose surface is provided with a two-side continuous pattern formed by combining at least one or multiple primitives; and a fixing part which fixes a marker to a surface of foreign matter in the acquired image with the mark part to facilitate algorithm recognition and marking. The method for recognizing a foreign matter marker in an image includes the steps of performing edge detection on a planar image to acquire an edge map in the planar image; and extracting all contours in the edge map. A certain number of alternative straight-line segments are determined using an algorithm; a region is determined according to the position of each alternative straight-line segment; and finally the region of the approximate area above or below the marker is used.

Abstract: A method for improving the gas recovery of a natural gas hydrate reservoir by using artificial impermeable layers is described. Artificial impermeable layers are formed by injecting cement slurry into the permeable overburden and underburden layers. When depressurization exploitation is performed, a large amount of seawater can be effectively blocked from entering a hydrate layer, and a production pressure difference between the hydrate layer and the production well is effectively increased, so that a hydrate decomposition rate and the gas recovery are improved.

Abstract: An LED filament and lamp, and a manufacturing process of an LED filament the LED filament includes a PCB substrate and multiple LED chips, connecting circuits are provided on the PCB substrate, a positive pin and a negative pin are provided at two ends of the connecting circuit, respectively, and each LED chip is electrically connected to the connecting circuit; and a fluorescent glue layer is provided outside the PCB substrate and the LED chips, and the positive pin and the negative pin are exposed from the fluorescent glue layer. The LED lamp includes the LED filament. The manufacturing process of an LED filament enables manufacturing an LED filament. For the LED filament and lamp, and the manufacturing process of an LED filament, a PCB substrate is used as a base material, connecting circuits are fabricated on the PCB substrate, the connecting circuits are directly used for connecting LED chips.

Abstract: The present invention relates to ligation and/or assembly of nucleic acid molecules. Particularly, a double-stranded target nucleic acid having overhangs of at least one nucleotide is ligated with another nucleic acid molecule capable of forming a stem-loop structure with an overhang of at least one nucleotide. The invention is suitable for tagging nucleic acid molecules. In specific embodiments, the overhangs can be produced by chemical cleavage of phosphorothioate-modified nucleic acid molecules. The invention further relates to the amplification of the ligated product, and using the resultant amplicon for assembly of multiple nucleic acid fragments.

Abstract: A geothermal energy system utilizes a Catherine Wheel that spins around an axle to produce power. The Catherine Wheel has a plurality of wheel arms extending radially out from the axle. A fin portion extends from the radial portion at an offset angle, to an exhaust end. A first working fluid, such as supercritical carbon dioxide flows through an arm conduit within the wheel arm and second working fluid, such as a simply hydrocarbon mixes with the first working fluid and both flow through a turbine. The turbine is configured within the wheel arm conduit and it turns as the combined working fluids vaporize. The fluids are expelled and the second working fluid may be condensed and recirculated while the first working fluid may be expelled back into a geothermal reservoir. The geothermal energy system efficiently and effectively produces power by using geothermal energy to vaporize a hydrocarbon.

Abstract: A smart Internet high-definition scanner with laser correction is provided. The scanner may include a calibration laser emission unit located above a scanning area, an image collection unit and an image processing unit. This scanner may collect an image of a page of an unfolded book by using a camera (image collection unit) and a plurality of laser beams directed toward an open book from the calibration laser emission unit.

Abstract: Provided is a method for flattening a laser-based image of a curved book page, which is applicable to a scanning platform and an image acquisition device and a laser generation device fixed above the scanning platform. The flattening method includes the following steps of: opening a book on a horizontally arranged scanning platform, and emitting a laser beam to a curved book page to be scanned, the laser beam forming a plurality of laser lines parallel on the curved book page; acquiring a horizontal image of the curved book page having the parallel laser lines, and acquiring a 2D planar coordinate (x, y) of each pixel on the laser line in the horizontal image; calculating, according a y-coordinate in the 2D planar coordinate, a y-coordinate of an intersection line of the laser beam and the scanning platform, and an intersection angle of the laser beam and the scanning platform, and by means of a tangent function, a height of each pixel on the laser line, i.e.

Abstract: A marker for occluding a foreign matter in an acquired image contains a mark part whose surface is provided with a two-side continuous pattern formed by combining at least one or multiple primitives; and a fixing part which fixes a marker to a surface of foreign matter in the acquired image with the mark part to facilitate algorithm recognition and marking. The method for recognizing a foreign matter marker in an image includes the steps of performing edge detection on a planar image to acquire an edge map in the planar image; and extracting all contours in the edge map. A certain number of alternative straight-line segments are determined using an algorithm; a region is determined according to the position of each alternative straight-line segment; and finally the region of the approximate area above or below the marker is used.

Abstract: Disclosed is a method for simulation of a microscopic flow of pre-crosslinked gel suspension liquid in a porous medium, including the steps of: establishing a simulation area of the porous medium based on a two-dimensional core CT slice image and subjecting the simulation area to numerical grid discretization; setting initial position and original shape of pre-crosslinked gel particles to generate virtual boundary mark points; marking the type of numeric grid nodes; calculating the force of the fluid on each virtual boundary mark point according to the momentum change of the numerical grid nodes on the boundary of the pre-crosslinked gel particles; calculating the contact force on each virtual boundary mark point using a particle contact action model; calculating the bending recovery force and the stretching recovery force for each virtual boundary mark point based on the current shape and original shape of the pre-crosslinked gel particles; and calculating the velocity and displacement of each virtual bounda

Abstract: The example of implementation of the invention provides a method used for exploiting a natural gas hydrate reservoir and belongs to the field of natural gas hydrate reservoir exploitation. The method mainly comprises a heat-injecting vertical well and a production horizontal well. The production horizontal well is opened in a segmental manner. In combination with natural gas hydrate heat exploitation, heat flow injected into the vertical well is enabled to heat different positions of the natural gas hydrate reservoir to increase a recovery ratio of the natural gas hydrate reservoir. The method specifically comprises the steps of injecting a heat flow into the natural gas hydrate reservoir by utilizing the vertical well to promote decomposition of the hydrate; firstly opening a horizontal branch of the horizontal well fully and keeping depressurization exploitation.

Abstract: The present invention discloses a smart Internet high-definition scanner with laser correction, which comprises a scanning area for placing a book to be scanned; a calibration laser emission unit located above the scanning area, an image collection unit and an image processing unit; wherein the calibration laser emission unit emits a laser beam to the book unfolded and placed on the scanning area, and forms a laser line on a surface of an opened page; the image collection unit collects a planar image of the bent page with the laser line; by establishing a three-dimensional coordinate system, and according to coordinates of pixel points of the laser line and an intersection angle between the laser beam and a plane of the scanning area, height coordinates of each pixel point on the laser line in the planar image in the three-dimensional coordinate system is obtained through calculation, a 3D curve of the laser line in the three-dimensional coordinate system is restored, and a real 3D curved surface of the page